Heart Rate Variability in the Human Transplanted Heart: Nonlinear Dynamics and QT vs RR-QT Alterations during Exercise Suggest a Return of Neurocardiac Regulation in Long-term Recovery M. MEYER, 3 C. MARCONI, 2 G. FERRETTI, 1 R. FIOCCHI, 4 P. CERRETELLI, 2 AND J.E. SKINNER 5 1Department de Physiologie, CMU, Geneve, Switzerland; 2Instituto di Tecnologie Biomediche Avanzate, CNR, Milano, Italy; 3Department of Physiology, Max Planck Institute f. exp. Medicine, Gottingen, Germany; 40spedali Riuniti, Bergamo, Italy; 5Torts Gap Institute, Bangor, PA, USA Abstract--Rationale. Functional reinnervation of the transplanted human heart by the autonomic nervous system has not been demonstrated. A lack of autonomic control of the transplanted allograft is reflected by an increased resting heart rate, a sluggish heart rate response to dynamical exercise and a reduced heart rate variability. Recent evidence sug- gests that a measure of deterministic chaos in the heartbeat interval series (point correlation dimension, PD2i) is superior to the conventional power spectrum or other stochastic mea- sures in detecting changes in the mechanism underlying heartbeat generation. Methods. The PD2i is based on the presumption that the variability is determined and patterned, whereas the stochastic measures all assume that the variability is around a stationary mean and is noise. The PD2i reconstructs the degrees of freedom (number of independent vari- ables) in the system that generates the time series examined, and does this irrespective of whether the system is stochastic or deterministic and is stationary in time. Results. PD2i was determined for heartbeat intervals (RR, ECG digitized at 1200 Hz; supine posture) of 23 heart transplant recipients (HTR: 9 adults, 14 children; 0.04-7.7 years after transplanta- tion) and 21 healthy control subjects (CTL; 13 adults, 8 children). The PD2i (+/- SD) averaged 5.4 +/- 0.7 for the CTL adults and 5.4 +/- 0.6 for the CTL children. Mean PD2i was reduced after transplantation to 1.1 +/- 0.1 in 6 HTRs recorded within 1 year after surgery; in one HTR recorded 2 weeks after surgery the mean PD2i was 3.7. Between 1 to 2 years PD2i was found increased in 2 of 3 subjects and between 2 to 8 years it was increased in 13 of 13, but not to control levels. In normal hearts the QT subinterval of each heartbeat cycle is associated with inotropy and the RR-QT remainder with chronotropy (i.e., the dyastolic interval during which RR is primarily regulated). To examine more closely the residual and returning heartbeat dynamics of the HTR subjects, these subinter- val series were examined during mild exercise (40 to 90 Watts) and its recovery. In recent HTRs, resting QT and RR-QT were moderately reduced and modulated by exercise and recovery, but with an approximate 100 beat latency. In long-term (7-8 years) HTR subjects there was a rapid and larger response to exercise/recovery, but compared to normal the range was smaller and the complexity of the subinterval trajectories in time was simpler. Address for correspondence: James E. Skinner, Totts Gap Medical Research Laboratories, Inc., 1430 Totts Gap Road, Bangor, PA, 18013; email: tgap@epix.net. Integrative Physiological and Behavioral Science, October-December, 1996, Vol. 31, No. 4, 289-305. 289