1 Page 1-6 © MAT Journals 2019. All Rights Reserved Journal of Molecular Pharmaceuticals and Regulatory Affairs Volume 1 Issue 2 Antimicrobial Activity Analysis of Cefixime with Antacid, Essential Metal and Interaction In Vitro study by Spectrophotometric Method Umme Israt Jahan 1 , Md. Shahidul Islam 2 * Student 1, Assistant Professor 2 Department of Pharmacy, University of science and Technology Chittagong (USTC), Chattogram, Bangladesh Email: *s_i_liton@yahoo.com DOI: http://doi.org/ 10.5281/zenodo.2906042 Abstract The present research work describes of interaction and complexion studies of Cefixime with essential metal & antacid and also investigation of antimicrobial activity of Cefixime. Cefixime included the third generation drug of cephalosporin. We know cephalosporin is very active against a wide range of gram positive as well as negative bacteria. In vitro analysis, Cefixime must be interacted with metal like Mg & antacid like CaCO 3 . At pH 7.4, this study was performed in different ratios of Cefixime with metal and antacid both at room temperature 25 0 C. by this study, it is investigated that drug Cefixime is complexed with metal as well as antacid which is confirmed by job’s plot . This experiment was carried out by using ultra violet spectrophotometer. The microbial sensitivity test is important to know whether there is any change in the effectivity of Cefixime after the interaction with metal and antacid. To observe the sensitivity in the experiment staphylococcus aureus was used. There was a remarkable change in the effectivity of Cefixime and its complexes. This research work confirms that there was interaction between Cefixime with Metals Mg 2+ and antacid CaCO 3 which was confirmed by Jobs plot method and by antimicrobial investigation. By antimicrobial investigation it was observed that the zone of inhibition of the drug Cefixime with metal Mg 2+ and antacid CaCO 3 reduced from 14mm to 12mm and 1mm respectively. Keywords: Cefixime, staphylococcus aureus, Antimicrobial activity, Interaction, job’s Plot. INTRODUCTION A drug interaction is said to occur when the effects of one drug are changed by the presence of another drug, herbal medicine, food, drink or by some environmental chemical agent. These unwanted and unsought-for interactions are adverse and undesirable but there are other interactions that can be beneficial and valuable, such as the deliberate co-prescription of antihypertensive drugs and diuretics in order to achieve antihypertensive effects possibly not obtainable with either drug alone. The mechanisms of both types of interaction, whether adverse or beneficial, are often very similar [1]. There are different types of drug interactions: Drug- drug interact ions, Drug-herbal interact ions, Food-drug interact ions etc. [2]. Drug interactions are complex and chiefly unpredictable. A known interaction may not occur in every individual. This can be explained because there are several factors that effects the likelihood that a known interaction will occur [3]. Zinc is an important co-factor for several enzymatic reactions in the human body, vitamin B 12 has cobalt atom and its core, and hemoglobin contains iron. Likewise Cu, Mn, Se, Cr, Mo are all trace elements, which are important in the human diet. Another subset of metals includes those used in therapeutically in medicine, Al, bi, Au, Ga, Li and Ag are all part of medical