American Journal of Analytical Chemistry, 2018, 9, 162-170 http://www.scirp.org/journal/ajac ISSN Online: 2156-8278 ISSN Print: 2156-8251 DOI: 10.4236/ajac.2018.93014 Mar. 15, 2018 162 American Journal of Analytical Chemistry Development and Validation of a Spectrofluorimetric Method for the Assay of Tetracycline in Capsules Lucien Murhula Namegabe 1* , Serigne Omar Sarr 2,3 , Yérim Mbagnick Diop 2,3 1 Department of Galenic Pharmacy, Official University of Bukavu, Bukavu, Democratic Republic of the Congo 2 Analytical Chemistry and Food Sciences Laboratory, Cheikh Anta Diop University, Dakar, Senegal 3 National Medecines Control Laboratory, Dakar, Senegal Abstract The purpose of this study is to develop and validate a method for the analysis of tetracycline capsules by spectrofluorimetry. A pH 9 borate buffer was used as diluent of tetracycline after reaction with magnesium salt at the excitation wavelength of 372 nm and 516 nm of emission. A linear response was ob- served between 0.25 μg/mL and 1.5 μg/mL with a correlation coefficient (R) of 0.9998. The detection and quantification limits found are 0.0125 μg/mL and 0.0412 μg/mL respectively. The proposed method proved trueness with a re- covery between 99.88% and 101.10%. The relative standard deviations of re- peatability and intermediate precision found ≤2.88% reflected a good preci- sion of the method. The proposed method is therefore valid within the limits of 90% to 110%. The proposed method was applied to the quality control of 9 tetracycline samples from market and gave results in accordance with the pharmacopoeia standards. Keywords Validation, Spectrofluorimetry, Tetracycline 1. Introduction Tetracycline hydrochloride (THC) is a broad-spectrum polyketide antibiotic produced by the genus Streptomyces. It exerts a bacteriostatic effect on bacteria by reversibly binding the 30S ribosomal subunit of bacteria and blocking the binding of tRNA to the ribosome acceptor site. It also binds to some extent to How to cite this paper: Namegabe, L.M., Sarr, S.O. and Diop, Y.M. (2018) Develop- ment and Validation of a Spectrofluorime- tric Method for the Assay of Tetracycline in Capsules. American Journal of Analytical Chemistry, 9, 162-170. https://doi.org/10.4236/ajac.2018.93014 Received: February 7, 2018 Accepted: March 12, 2018 Published: March 15, 2018 Copyright © 2018 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access