Journal of Surgery 2015; 4(1-1): 5-10 Published online September 7, 2015 (http://www.sciencepublishinggroup.com/j/js) doi: 10.11648/j.js.s.2016040101.12 ISSN: 2330-0914 (Print); ISSN: 2330-0930 (Online) Effect of Honey versus Icodextrin on Adhesion Reformation after Adhesiolysis: an Experimental Study in Rats Aly Saber 1 , Mohammed H. Shekidef 2 , Aya Aly Saber 1, * 1 Port-Fouad General Hospital, Port-Fouad, Egypt 2 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Ismailia, Suez Canal University Email address: shekidef77@yahoo.com (Mohammed H. Shekidef), Draya1207@gmail.com (A. A. Saber) To cite this article: Aly Saber, Mohammed H. Shekidef, Aya Aly Saber. Effect of Honey versus Icodextrin on Adhesion Reformation After Adhesiolysis: An Experimental Study in Rats. Journal of Surgery. Special Issue: Abdominal Surgery: Toward the Best. Vol. 4, No. 1-1, 2015, pp. 5-10. doi: 10.11648/j.js.s.2016040101.12 Abstract: Introduction: Although the process of de novo adhesion formation after operation has been fairly well established, adhesion reformation in a previously affected zone is still poorly understood. Honey was proved effective as an anti-adhesive agent in experimental animals. The aim of the present experimental study is to investigate the effect of HONEY on adhesion reformation prevention when compared with ICODEXTRIN in rats. Materials & Methods: 75 healthy male Sprague–Dawley rats having average weight250–300 g were divided into three groups for study: [25 rats for each], Honey, Icodextrin and control groups. Laparotomy was done carefully for not to disturb any adhesion could be attached to the previous laparotomy incision. Any adhesion band was dissected and divided gently and smoothly with sharp maneuver using fine surgical scissors. In case of groups A and B, three ml of Honey and Icodextrin were instillated, using sterile syringes; into the abdominal cavity before completing laparotomy wound closure to prevent escape of the material outside the peritoneal cavity. Results: Total adhesion score among the three groups showed statistically significant difference using the ANOVA test but when comparing group A and B,the difference is considered to be not statistically significant. Conclusion: Both icodextrin and honey has beneficial effect on reduction of the extent and severity of intraperitoneal adhesion reformation in our rat model for colonic anastomoses. Honey surpasses icodextrin regarding the total adhesion score and the score per animal. Keywords: Adhesion Reformation, Honey, Icodextrin, Rats 1. Introduction Intra-abdominal adhesion formation and reformation after surgery are still unavoidable events in spite of modern surgical techniques [1]. Operative trauma and surgical manipulation as well as ischaemia of the traumatized tissues induce peritoneal mesothelial damage which is considered the trigger of adhesion formation [1,2]. Although the process of de novo adhesion formation after operation has been fairly well established, adhesion reformation in a previously affected zone is still poorly understood [3 ]. After laparotomy, postoperative adhesion formation is reported to occur nearly in 100% of patients and after laparoscopic surgery, the adhesion reformation rate can be as high as 97% [4]. For prevention of formation and reformation of post-operative intra-abdominal adhesions a variety of surgical techniques and adjuvants have been proposed. Anti-adhesive agents can be classified as pharmacological agents and intra-peritoneal barriers [5,6]. Icodextrin is a non-viscous, iso-osmotic, clear solution proven safe and effective as an anti-adhesion device when used during surgery and as post-operative instillate providing a fluid reservoir in the peritoneal cavity with a prolonged residence time of up to 4 days. It acts to temporarily separate peritoneal surfaces through the process of hydroflotation, thereby minimizing tissue apposition during the critical period of fibrin formation and mesothelial regeneration following surgery, and thus providing a barrier to adhesion formation[7] and Honey was proved effective as an anti-adhesion and antibacterial agent in experimental animals [1,8]. The aim of the present experimental study is to investigate the effect of HONEY on adhesion reformation prevention when compared with ICODEXTRIN in rats.