Complete Genome Sequence and Annotation of the Staphylococcus aureus Strain HG001 Isabelle Caldelari, a Béatrice Chane-Woon-Ming, a Céline Noirot, b Karen Moreau, c Pascale Romby, a Christine Gaspin, b Stefano Marzi a Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, UPR 9002, Strasbourg, France a ; UBIA & PF GenoToul Bioinfo, INRA, Castanet Tolosan, France b ; Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, INSERM U1111, CNRS UMR 5308, CIRI, Lyon, France c ABSTRACT Staphylococcus aureus is an opportunistic Gram-positive pathogen responsi- ble for a wide range of infections from minor skin abscesses to life-threatening diseases. Here, we report the draft genome assembly and current annotation of the HG001 strain, a derivative of the RN1 (NCT8325) strain with restored rbsU (a positive activator of SigB). S taphylococcus aureus is an opportunistic Gram-positive pathogen that is present in one-third of the population on skin and in nostrils (1) as commensal bacteria, but it is also responsible for a wide range of infections from minor skin abscesses to life-threatening diseases, such as septicemia and toxic shock syndrome (2). S. aureus represents a major health problem due to the insurgence of antibiotic-multiresistant strains in hospital and community settings. Many complete S. aureus genome sequences are available in the NCBI database (currently 116). These genomes were obtained from isolates from patients with sepsis and also from laboratory strains or have been generated with molecular tools. Several transcriptomic analyses have been conducted under various conditions by different laboratories (3–8) using the standard model HG001 strain, a derivative of the RN1 (NCT8325) strain with repaired rbsU (a positive activator of SigB). The strain still contains a mutation at tcaR, a transcriptional regulator involved in teicoplanin susceptibility and biofilm (9). Nevertheless, a complete genome sequence of this commonly used S. au- reus laboratory strain is not available. Here, we present the draft genome sequence of the reference strain HG001. Chromosomal DNA was extracted using the Gentra Puregene kit (Qiagen) following the manufacturer’s guidelines. Whole-genome sequencing was conducted with the PacBio RS platform (GATC, Biotech SARL, Germany). Two single-molecule real-time (SMRT) cells were used to sequence the genome (mean coverage of 78 times the genome), which was assembled de novo from 6 contigs with the SMRT portal (PacBio). The pseudomolecule was constructed by referring to the NCTC8325 genome sequence (GenBank accession no. CP000253, NCBI reference sequence no. NC_007795). The Seaview tool (10) has been used to manually align contigs. The final genome assembly consists of a 2,819,767-bp-long DNA molecule. Genome annotation has been done using Prokka v1.10 (11), which predicted 2,617 coding sequences (CDS), 62 tRNAs, 15 rRNAs, 1 transfer-messenger RNA (tmRNA), and 59 other noncoding RNAs. This anno- tation has been manually curated, with the addition of 4 more peptides and 418 noncoding RNAs from the Staphylococcal Regulatory RNAs Database (http://srd .genouest.org/raw//sRNA/sRNA_NCTC8325.gff) (12) and is available for download (http://www-ibmc.u-strasbg.fr/spip-arn/spip.php?rubrique152). Accession number(s). The complete genome sequence for the HG001 strain has been deposited in GenBank under the accession no. CP018205. Received 23 June 2017 Accepted 27 June 2017 Published 10 August 2017 Citation Caldelari I, Chane-Woon-Ming B, Noirot C, Moreau K, Romby P, Gaspin C, Marzi S. 2017. Complete genome sequence and annotation of the Staphylococcus aureus strain HG001. Genome Announc 5:e00783-17. https://doi.org/10.1128/genomeA.00783-17. Copyright © 2017 Caldelari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Christine Gaspin, cgaspin@toulouse.inra.fr, or Stefano Marzi, s.marzi@ibmc-cnrs.unistra.fr. PROKARYOTES crossm Volume 5 Issue 32 e00783-17 genomea.asm.org 1