Int. J. Curr. Res. Biosci. Plant Biol. (2018) 5(10), 7-13 V. Ouedraogo et al. (2018) / Acacia seyal Del Bark Extract Reduces Quorum Sensing –Controlled Virulence Factor Production and Biofilm Formation in Pseudomonas aeruginosa PAO1 7 International Journal of Current Research in Biosciences and Plant Biology Volume 5 ● Number 10 (October-2018) ● ISSN: 2349-8080 (Online) Journal homepage: www.ijcrbp.com Original Research Article doi: https://doi.org/10.20546/ijcrbp.2018.510.002 Acacia seyal Del Bark Extract Reduces Quorum Sensing –Controlled Virulence Factor Production and Biofilm Formation in Pseudomonas aeruginosa PAO1 Vincent Ouedraogo 1 *, Ablassé Rouamba 1 , Moussa Compaoré 1 , Pierre A.E.D Sombié 1,2 and Martin Kiendrebeogo 1 1 Laboratory of Biochemistry and Chemistry Applied (LABIOCA), University Ouaga I Professor Joseph Ki-Zerbo, Burkina Faso 09 P.O Box 848 Ouagadougou 09 2 National Center of Scientific and Technological Research, Institute of Environment and Agricultural Research, Burkina Faso, 01 P.O Box 476 Ouagadougou 01 *Corresponding author. Article Info ABSTRACT Date of Acceptance: 15 September 2018 Date of Publication: 06 October 2018 Due to the various virulence factors produced and the growing resistance to antibiotics, Pseudomonas aeruginosa is the major cause of mortality in immunocompromised patients. Its pathogenicity regulated by the QS system lead to consider this mechanism of bacterial communication an opportunity for the control of infections caused by bacteria resistant to antibiotics. Thus, the ability of Acacia seyal bark extract to disrupt the cell-to-cell communication, to inhibit the production of virulence factors and biofilm formation was assessed in Pseudomonas aeruginosa PAO1. These investigations demonstrated that A. seyal bark extract inhibited significantly the production of QS- controlled virulence factors and biofilm formation without negatively affecting the growth of P. aeruginosa PAO1. These results suggest that A. seyal quenches the quorum sensing mechanism and could be a potent source for the research of new effective anti- QS drugs. Keywords Acacia seyal Biofilm Pseudomonas aeruginosa Quorum sensing Introduction The emergence of pathogenic bacteria resistance to antibiotics observe over the last decades has stimulated the search for news antibacterial drug with novel targets (Rasamiravaka et al., 2013). In recent years, the discovery of bacterial communication system which regulated the expression of virulence genes provided a new opportunity for the control of bacterial infections (Adonizio, 2008). This system known as QS is considered to be a key regulator mechanism in ecological adaptation and pathogenicity of bacteria (Bassler and Losick, 2006). This system is based, in