Original Article ISSN (Online): 2350-0530 ISSN (Print): 2394-3629 International Journal of Research - GRANTHAALAYAH January 2022 10(1), 34-46 How to cite this article (APA): Fırat, F., and Aladağ, T., (2022). Comparative Effects of Boric Acid and Resveratrol on MCF-7 Breast Cancer Cells Metastatic Behaviour. International Journal of Research - GRANTHAALAYAH, 10(1), 34-46. doi: 10.29121/granthaalayah.v10.i1.2022.4460 34 COMPARATIVE EFFECTS OF BORIC ACID AND RESVERATROL ON MCF-7 BREAST CANCER CELLS METASTATIC BEHAVIOUR Fatma Fırat 1 , Tuğçe Aladağ 2 1, 2 Department of Histology and Embryology, Faculty of Medicine, Afyonkarahisar Health Sciences University Afyonkarahisar, Turkey. Received 01 December 2021 Accepted 26 December 2021 Published 31 January 2022 Corresponding Author Tuğçe Aladağ, tugcealadagg@hotmail.com DOI 10.29121/granthaalayah.v10.i1.2022 .4460 Funding: This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors. Copyright: © 2022 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT Motivation/Background: The metastasis of tumor cells consists of steps such as epithelial-mesenchymal transition, transendothelial migration and the formation of metastatic colonization. E-Cadherin and vimentin are main proteins associated with EMT, whereas MMP-9 is associated with migration. Method: We aimed to investigate effects boric acid and resveratrol comparatively on metaatatic behaviors on MCF-7. 30μM boric acid and 50μM resveratrol were administered to BA, BA+RES, and RES groups 48hours. Cells stained immunocytochemically by Anti-E-Cadherin, Anti-Vimentin, and Anti-MMP-9 antibodies and H-Score analysis carried out and migration analyzed by woundhealing, morphologically. Results and Conclusions: It’s been observed that boric acid doesn’t affect the EMT capability of the MCF-7 cells in terms of E-Cadherin and vimentin expression; whereas it’s affected migration both by decreasing the MMP-9 expression and also by inhibiting migration. Keywords: Boric Acid, MCF-7 Breast Cancer, Epithelial-Mesenchymal Transition, Metastasis, Resveratrol. 1. INTRODUCTION Breast cancer is a type of cencer which has the highest mortality rates and is the most common cancer in women Global Cancer Observatory (2021). There are novel therapeutic approaches being developed in breast cancer treatment nowadays Mahjoubin-Tehran et al. (2020). Despite this, there is still need for effective methods in terms of cencer treatment. In general, deaths based on cancer is mainly caused by metastasis Dillekås et al. (2019). Banfalvi (2012) believes that the development of breast cancer is mainly metastasis rather than the primary tumor and states that the main reason of death caused by breast cancer is tumor invasion and metastasis Banfalvi (2012). 10-15% of patients usually have metastasis during the three years following the first treatment. Metastasis includes stages such as local invasion (Epithelial-Mesenchymal Transition (EMT)), intravasation, survival in the circulation, extravasation (transendothelial migration (TEM/diapedesis)), the development of micrometastasis and the development of metastatic colonization Reymond et al. (2013). Metastasis in cancer cells is a complex process directed by a great number of biochemical and molecular factors. Although this process and the mechanisms of action are not completely enlightened, the chance to eliminate the disease will arise if the cancer cells can be stopped in the invasion stage.