Available online on www.ijppr.com International Journal of Pharmacognosy and Phytochemical Research 2017; 9(7); 1029-1034 doi: 10.25258/phyto.v9i07.11176 ISSN: 0975-4873 Research Article *Author for Correspondence: drbeenajose@gmail.com Antioxidant, Antimicrobial and Cytotoxic Activities of Alcoholic Leaves Extracts of Spatholobus parviflorus (Roxb.Ex Dc.) Kuntze Jesy E J 1 , Beena Jose 2* 1 R&D Centre, Bharathiar University, Coimbatore-641046, India. 2 Depatrment of Chemistry, Vimala college,Thrissur,Kerala-680009, India. Received 23 rd March, 17; Revised 28 th June, 17, Accepted 14 th July, 17; Available Online 25 th July, 2017 ABSTRACT Objective: To investigate the total phenolics, antioxidant , antimicrobial and cytotoxic, activities of ethanolic and methanolic leaves extracts of Spatholobus parviflorus (Roxb.ex Dc.) kuntze. Methods: The current study was focussed on broad parameters namely total phenolics, phytochemical analysis, gas chromatography-mass spectrometry analysis and antioxidant properties in order to characterize the alcoholic extracts of Spatholobus parviflorus as apotential free radical quencher. Results: The phytochemical screening of alcoholic extracts of Spaholobus parviflorus showed the presence of various secondary metabolites. Spatholobs parviflorus was proved to be an effective radical scavenger in all antioxidant assays. The gas chromatographic- mass spectrometry analysis confirmed the presence of bioactive compounds in varying percentage. Conclusion: These results suggest that the ethanolic extract of Spaholobus parviflorus has potential of antioxidant,antibacterial and cytotoxic activity that support the ethnopharmacological uses of this plant.The remarkable activity showed by the plant extract could be attributed to the synergic effect of the active compounds present in it. Keywords: total phenolics, cytotoxic activity, antioxidant, antimicrobial activity, Spatholobus parviflorus. INTRODUCTION Medicinal plants are a source of great economic value all over the world. Nature has given us a very rich botanical wealth and large number of diverse types of plants grows in different parts of the country. Ayurveda, Unani and Siddha are systematically used nearly 1500 plants in indigenous system of medicine. Medicinal plants are the oldest existing complete medical system in the world. Use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicines contain a wide ratio of substances that can be used to treat chronic as well as communicable diseases 1 . Plants are used medicinally in different countries and are a source of many potent and powerful drugs. According to World Health Organization (WHO) more than 80% of the world's population relies on traditional medicine for their primary healthcare needs. Medicinal plants contain large varieties of chemical substances which possess important therapeutic properties that can be utilized in the treatment of human diseases. The studies of medicinal plants used in folklore remedies have attracted the attention of many scientists in finding solution to the problems of multiple resistances to the existing synthetic antibiotics. Most of the synthetic antibiotics now available in the market have major setback due to the multiple resistance developed by pathogenic micro-organisms against their drugs 2 . Modern technique and pharmacological screening procedure results new plant drugs usually find their way into modern medicines. Now a day’s maximum number of plant are being screened for their possible pharmacological value. The plant kingdom still hold many plant species containing substance of medicinal value which have yet to be discovered. The medicinal value of plants lies in some chemical substances that produce a definite physiologic action on the human body. The most important of these phytocompounds of plants are alkaloids, flavonoids, tannins, terpenoids and phenolic compounds. Spatholobus parviflorus (Roxb.ex Dc.) Kuntze (Fabaceae) is a rare endemic threatened strong woody climber which is less evaluated for its medicinal properties. These woody climbers are widely distributed in a wide geographic range from Nepal, Bhutan and India. They are mainly seen in semi-evergreen and moist deciduous forests and also in sacred groves. In Kerala leaf paste is used to treat conjuctivities 3 . In northern Thailand the leaves and stem of this species are boiled with dicranopteris and used as a liquid to apply to broken bones as an analgesis 4 . Gum extracted from the wood, fibre from the bark and oil from the seeds is reputed to have economic use in Bangladesh 5 . GC- MS analysis of ethanol extracts of leaves revealed that Lupeol(28.70%) as the major component followed by the Hexadecanoic acid, [3,7,11,15]-tetramethyl-2-hexadecen- 1-ol,2-tridecnol-1-ol, phytol ,1- heptatriacotanol 6 .According to the recent published reports, triterpenoid Lupeol has been shown to exhibit various pharmacological activity under in vitro and in vivo conditions. These include its antioxidant, antimicrobial and anticancer activities 7 .