Qatar Univ. Sci. J. (1995), 15 (1): 51 • 55 CONSTITUENTS OF PLANTS GROWING IN QATAR: PART XXVIII. CONSTITUENTS OF CISTANCHE PHELYPAEA By T. DEYAMA*, K. YAHIKOZAWA*, H.S. AL-EASA** AND A.M. RIZK** *Central Research Laboratories, Yomeishu Seizo Co., Ltd., 2132-37. Nakamminowa Minowa-machi, Kamuna-gum, Nagano 394-46. **Department of Chemistry, Faculty of Science, University of Qatar, Doha. Qatar. : 4WI )1 ,:r 4..... 1;.) ..\.:-oJ;.P-, J_r.lulS J.:.:..:-1-", . Key Words: Cistanche phelypaea, Orobanchaceae, Echinacoside, Tubuloside A, Tubuloside E,Acteoside, 2'-Acetylacteoside, 6Deoxycatalpol, Gluroside, Ajugol, Syringin, ABSTRACT Ten compounds were isolated from the methanolic extract of the aerial parts of Cistanche phelypaea (Orobanchaceae) and identified as echinacoside (1), tubuloside A (2), tubuloside E (3), acteoside (4), 2'-acetylacteoside (5), 6-deoxycatalpol (6), gluroside (7), ajugol (8), sy- ringin (9) and -sitosterol ( 1 0). INTRODUCTION Cistanche phelypaea (L.) Cout. (Orobanchaceae), a par- asitic plant grows on Arthrocnemun glaucum and Seidlitzia rosmarinus, is a common species in southern Qatar [1 ]. The whole plant is medicinally used as a remedy for di- arrhea, a tonic in speratorrhea impotence and a cataplasm against bruises [2]. Phenylethanoid glycosides have been isolated from Cistanche tubulosa [3,4] C. salsa [5,6] and C. deserticola [7]. Melek, et. at. [8] reported the isolation of four phenylethanoid glycosides from C. phelypaea grow- ing in Egypt, theses are as follows: tubuloside A, acteoside, 2'-acetylacteoside and pheliposide [8]. The pharmacolog- ical studies for the same plant showed that the acute toxic- ity was minimal. Analgesic, antipyretic and diuretic activ- ities of the extract were significant only at large doses[8]. 51 EXPERIMENTAL Plant material Cistanche phelypaea (aerial parts) was collected from north Al-Markhya (3 Km north of Doha), Qatar in March. The plant was identified by Prof. K. H. Batanouny. Extraction procedure The plant was air dried, powdered, extracted in a soxhlet with MeOH and freed from solvent under vacuo, yielding (200 g) of crude extract. The extract was then suspended in distilled water and the suspension was extracted with ether, EtOAc and n-BuOH yielding 1.59 g, 0.95 g and 2.0 g ex- tracts respectively.