Demonstration of Muscle Fibre Types Using Masson Trichrome Stain from Deltoideus Muscles of One-humped Camel (Camelus Dromedarius) Hena SA 1* , Sonfada ML 1 , Shehu SA 1 , Jibir M 2 , Bello A 1 and Wiam IM 3 1 Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria 2 Department of Animal Science, Faculty of Agriculture, Usmanu Danfodiyo University, Sokoto, Nigeria 3 Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria * Corresponding author: Hena SA, Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria, Tel: 2348060524623; E-mail: sundayhena@yahoo.co.uk Received date: November 30, 2016; Accepted date: January 11, 2017; Published date: January 20, 2017 Copyright: © 2017 Hena SA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract This study involved the use of 25 male camels (Camelus dromedarius) each within the ages of 6 months, 1 year, 3 years, 5 years and 7 years, which were purchased from Sokoto Municipal Modern abattoir. 1 cm 2 of each deltoideus muscle sample was taken from the middle part of the muscle belly and fixed in Bouin’s solution for histological preparation using Masson trichrome according to standard procedure; the obtained slides were viewed using a miscroscope using different magnifications. The Masson trichrome stain revealed some collagen connective tissue fibres, and varying proportions and distribution patterns of muscle fibre types I, IIA and IIB. This study could help in advancing the course of knowledge in this study field and as well guide in clinical practice more especially during evaluation of muscular procedures and wound healing. Keywords: Demonstration; Muscle fbre; Masson trichrome; Deltoideus; Camel Introduction Te trichrome stain is one of the most utilized special stains in the histology and histopathology Laboratory. Most of the common uses for requesting a trichrome stain are liver biopsies, renal biopsies, dermatopathology, cardiac biopsies and muscle and nerve biopsies, and widely utilized techniques are the Masson, Gomori One Step, Martius Scarlet Blue and Mallory [1]. Te purpose of the trichrome stain is primarily to demonstrate collagen and muscle in normal tissue or to diferentiate collagen and muscle in tumors. It is also used to identify an increase in collagenous tissue or indicate fbrotic change in cirrhosis of the liver or in a renal disease such as pyelonephritis. Te trichrome stain is also used to distinguish tumors that have arisen from muscle cells and fbroblasts. Gomori’s trichrome is the trichrome stain of choice for distinguishing histological changes that occur in neuromuscular diseases [1]. Deltoideus is a muscle that lies partly on the triceps brachii in the angle between the scapula and the humerus, and partly on the infraspinatus and teres minor. It is named afer the Greek letter delta, which is shaped like an equilateral triangle, and it is divided into the scapular part and the acromial part [2]. Te Pars scapularis arises aponeurotically from the scapular spine, the vertebral border of the infraspinous fossa and the surface of the infraspinatus muscle, becoming feshy over the caudal border of the infraspinatus. It partly covers the origin of the long and lateral heads of the triceps brachii. Some of its fbres attach to the fascia covering the lateral head, while a strong fascial band on its deep face inserts on the deltoid tuberosity and on an area proximal to it [2]. Te pars acromialis arises from the acromion and inserts in common with the scapular part of the deltoideus [2,3]. Te main action of the muscle is to fex the shoulder joint and abduct the arm [4]. Camel is used as a beast of burden more especially with loads or strain being placed on them, the animal’s limbs and their musculatures are the structures that bear the strain for locomotion, traction and support. Due to the function of the deltoideus muscles in fexing the shoulder joint and abducting the arm; it was pertinent to study the anatomy of the muscle in the forelimb of this animal species. Tis will help in advancing the course of knowledge in this study area which could fnd application even in clinical practices as Masson trichrome stain could be used to diagnose or detect some muscular diseases. Materials and Methods Forelimbs from 25 male camels (Camelus dromedarius) and those of 25 male cattle (Zebu type) each within the ages of 6 months, 1 year, 3 years, 5 years and 7 years were purchased from Sokoto Municipal Modern abattoir. Te age of each animal was determined, prior to slaughter, using the methods of Dyce KM et al. [3] and Wilson RT [5], while evaluation to exclude any animal with musculoskeletal deformity or diseases was done through physical examination. Te live body weights of the animals were estimated using linear body measurement based on the formula of Yagil R [6]. Te limbs obtained were wrapped in clean polythene bags and transported in a clean cool box containing ice cubes to the Laboratory of the Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto-Nigeria, where the triceps brachii muscles were all carefully dissected out using the methods of Chibuzo GA [7] as slightly modifed by Sonfada ML [8] afer most of the connective tissues ensheathing the muscle were trimmed of. 1 cm 2 of each muscle sample was taken from the middle part of the muscle belly and fxed in Bouin’s solution for histological Hena SA et al., J Mol Histol Med Physiol 2017, 2:1 Research Article Open Access J Mol Histol Med Physiol, an open access journal Volume 2 • Issue 1 • 1000111 Journal of Molecular Histology & Medical Physiology M o l e c u l a r H i s t o l o g y & M e d i c a l P h y s i o l o g y