~ 1465 ~ Journal of Pharmacognosy and Phytochemistry 2019; 8(2): 1465-1468 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2019; 8(2): 1465-1468 Received: 16-01-2019 Accepted: 19-02-2019 Pranjali M Pore Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India RS Ingole Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India Madhuri Hedau Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India SW Hajare Department of Pharmacology and Toxicology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India KK Khose Department of Poultry Science, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India MV Ingawale Department of Poultry Science, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India Correspondence RS Ingole Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India Effect of Andrographis paniculata on hematobiochemical profile during monensin induced toxicity in Japanese quails Pranjali M Pore, RS Ingole, Madhuri Hedau, SW Hajare, KK Khose and MV Ingawale Abstract The present investigation was carried out to study the ameliorative effect of Andrographis paniculata on hematobiochemical profile during monensin toxicity in Japanese quails. At the end of 28 day quails fed with monensin @ 150 mg/kg of feed showed adverse effect on haematological profile of Hb, PCV, TEC, TLC, heterophil and lymphocyte count, whereas birds given monensin along with dried leaves powder of Andrographis paniculata showed dose dependant (@ 3g/kg and 5 g/ kg feed) beneficial effect. Monensin only toxicated birds showed significant increase in serum AST, creatinine, and phosphorus whereas birds given monensin along with dried leaves powder of Androgrphis paniculata showed significant decreased values and suggested beneficial property of plant during monensin toxicity. Increased serum calcium level in monensin treated birds showed restoration when given Andrographis paniculata along with it. Thus it is concluded that dried leaves powder of Andrographis paniculata showed hematinic, hepatoprotective and renoprotective effect @ 3g/kg and 5 g/ kg feed during monensin sodium toxicity in Japanese quails given @ 150 mg/kg in feed. Keywords: Andrographis paniculata, biochemical, hematology, monensin Introduction Monensin is a fermentation product of Streptomyces cinnamonensis and is the 1 st antibiotic used as an anticoccidials. Due to its broad spectrum activity, it acts on trophozoites and 1 st generation schizonts. Its activity is generally within first 2 days of life cycle of coccidian. It gives protection against all species of coccidia at 0.01 - 0.121% concentration in the feed. In commercial poultry farming monensin is used in commercially prepared feed @ 125 mg/kg in broiler chicken, @ 100 mg/kg in turkey and @ 73 mg/kg in quails as prophylactic dose. Monensin given at 20 to 50 percent over dosage may cause the first evidence of toxicity. Simultaneous applications of monensin and tiamulin (a pleuromutilin derivative used against mycoplasma infections) results in increased toxicity [1] . The monensin induced growth depression was recorded when diets contained lower amounts of crude protein [2] . The occurrences of monensin toxicity due to contaminated or improperly prepared feed have been reported in the chickens, turkeys and guinea fowl [3, 4, 5, 6] . Monensin Na + specific ionophore easily forms lipophilic complexes with other ions (monovalent cations) and results in an influx of Na + with corresponding efflux of H + and K + leading to an increase in intracellular Ca + . Oxidative stress produced due to monensin causes monensin toxicity [7] . The monensin toxicity primarily manifest as a neuromuscular dysfunction which can result in paralysis and death of toxicated birds with intermyofibrilar vacuolization, vacuolization in the epicardium, myofibrilar degeneration and necrosis of skeletal and myocardial muscle. The aerial parts, roots and whole plant of Andrographis paniculata have been used for centuries in Asia as traditional medicine for the treatment of various ailments. The leaf extract is a traditional remedy for the treatment of infectious diseases, fever-causing diseases, colic pain, loss of appetite and diarrhoea [8] . The chemical constituents of Andrographis paniculata are diterpene lactones, andrographolides, neoandrographolides and kalmeghin, several flavones and flavonoids [9] . The two main diterpenoids isolated from Andrographis paniculata are 14-deoxy-11, 12-didehydroandrographiloid [10] . Presence of these constituents could attribute to cardioprotective activity of Andrographis paniculata by antioxidant, free radical scavenging and antilipid peroxidation property. Literature reviewed however did not reveal information relating to experimental studies of use of Andrographis paniculata plant during monensin toxicity in Japanese quail. Considering antioxidant and cardioprotective activity of Andrographis paniculata [11] the present study was conducted to evaluate the ameliorative