* Corresponding author: i.ghiglieno@unibs.it Characterisation of Franciacorta vineyard agroecosystem to support biodiversity and ecosystem services Isabella Ghiglieno 1,* , Anna Simonetto 2 , Elia Lipreri 1 , Giorgio Sperandio 1 , Stefano Armiraglio 3 , Ivo Rigamonti 4 , Pierluigi Donna 5 , and Gianni Gilioli 1 1 Università di Brescia - Dipartimento di Ingegneria Civile, Architettura, Territorio, Ambiente e di Matematica – Agrofood Lab, via Branze, 43, 25123 Brescia, Italy 2 Università di Brescia - Dipartimento di medicina molecolare e traslazionale – Agrofood Lab, viale Europa, 11, 25121 Brescia, Italy 3 Museo Civico di Scienze Naturali - Sez. di Botanica, via Ozanam 4, 25128 Brescia, Italy 4 Università degli Studi di Milano – Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente, via Celoria 2, 20133, Milano, Italy 5 Sata Studio Agronomico S.r.l. - S.t.p., Piazza della Loggia 5, 25121, Brescia, Italy Abstract. Vineyard is a multifunctional ecosystem associated to a multitude of environmental benefits and ecosystem services. Despite the increased research efforts on the analysis of biodiversity patterns and ecosystem services in vineyard, the lack of approaches to multifunctionality suggests to develop integrated approaches allowing to manage the complexity of vineyard landscape. The present study introduces an innovative methodology aimed at developing a unitary framework able to describe vineyard ecosystems biodiversity considering both local (morphological characteristics, internal ecological infrastructure, and management) and landscape (land-use) components. The case of Franciacorta wine-growing area is described considering a total of 112 ha located in three wine companies. Four informative layers related to different components of Franciacorta territory were defined: i) morphology; ii) internal ecological infrastructure; iii) landscape composition; iv) management. This study provides a useful instrument to increase knowledge about vineyard system biodiversity and to protect ecosystem services provision in Franciacorta territory. The methodology applied can easily be extended to the whole wine-growing compartment. 1 Introduction Viticulture is among the oldest and most representative forms of agriculture, covering about 7.3 million hectares worldwide [1]. Vineyards strongly shape the appearance of landscapes in a territory [2, 3]. The vineyard is a multifunctional ecosystem [4] associated with a multitude of ecosystem services, performing important economic, cultural, and ecological roles. Winkler et al. [5] identified 27 ecosystem services classes associated with vineyards; among them the most investigated resulted: cultivated crops, sequestration, pest control, disease control, scientific, and heritage, cultural services. Biodiversity represents a key actor in the provisioning of these ecosystem services in the vineyard ecosystem, both at local (vineyard and the areas adjacent to vineyards) and, more extensively, at landscape level [6]. In this context, research efforts on the relationships between biodiversity patterns and ecosystem services in vineyards increased [5]. However, most of the studies addressed specific issues related to biodiversity while few studies adopted approaches to multifunctionality in vineyards [4]. The complexity of the vineyard landscape imposes to perform innovative integrated approaches allowing to define a systemic analysis of vineyards agroecosystems [7], including both local and landscape elements, evaluated at different temporal and spatial scales. In the present study, an innovative methodology is proposed to develop a unitary framework able to describe vineyard agroecosystem's biodiversity considering all local (morphological characteristics, internal ecological infrastructure, and management) and landscape (land-use) components. The methodology is applied to the case study of the Franciacorta wine-growing area (Lombardy, Italy). Franciacorta is one of the most important Italian wine- growing regions for sparkling wine production. There, farmers are aware of issues related to biodiversity and eco-tourism represents an important resource. Results obtained from this preliminary study set the ground for specific monitoring activities addressing the main components of biodiversity, providing a useful instrument for wine growing companies and the whole Franciacorta territory to increase knowledge about vineyard agroecosystem and to protect ecosystem services provision. This case study represents a virtuous example for the wine-growing compartment promoting a © The Authors, published by EDP Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/). BIO Web of Conferences 44, 02003 (2022) https://doi.org/10.1051/bioconf/20224402003 CO.NA.VI. 2020