Journal of Food Research; Vol. 9, No. 4; 2020 ISSN 1927-0887 E-ISSN 1927-0895 Published by Canadian Center of Science and Education 1 Effects of Maturity on the Development of Oleic Acid and Linoleic Acid in the Four Peanut Market Types Lisa L. Dean 1 , Claire M. Eickholt 2, 3 , Lisa J. LaFountain 2 & Keith W. Hendrix 1 1 Market Quality and Handling Research Unit, USDA, ARS, SEA, Raleigh, NC, USA 2 Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA 3 General Mills Corporation, Minneapolis, MN, USA Correspondence: Lisa L. Dean, Market Quality and Handling Research Unit, USDA, ARS, SEA, Box 7624, NCSU, Raleigh, NC, USA. Tel: 1-919-515-9110. E-mail: Lisa.Dean@usda.gov Received: April 23, 2020 Accepted: May 27, 2020 Online Published: June 10, 2020 doi:10.5539/jfr.v9n4p1 URL: https://doi.org/10.5539/jfr.v9n4p1 Abstract The commercialization of high oleic peanut varieties with the fatty acids, oleic and linoleic present in a ratio greater than 9 has increased the shelf stability of many products containing peanuts significantly. With no visual traits to determine levels of the fatty acids present, mixing of the high oleic peanut types from the normal oleic types has been a problem in the peanut supply chain. This study investigated the effect of the development of the fatty acids in peanuts over their maturation with respect to the different market types (Runner, Viriginia, Spanish, Valencia) to determine if the maturation stage of the peanut could be responsible for the presence of normal oleic peanuts in lots of high oleic peanuts and thus decreasing the purity of the lots. Peanuts had different levels of the main fatty acids present as the oil content increased with maturation. Due to the presence of a natural desaturase enzyme in peanuts, oleic acid is converted to linoleic as the peanut develops resulting in a ratio of oleic acid to linoleic acid of 3 or lower in normal oleic peanuts. In peanuts from high oleic cultivars, the genes encoding for this enzyme are mutated or slow to develop. As this gene is activated in the later stages of peanut maturity, this study proves immature peanuts of the high oleic type may not have the proper ratios of oleic to linoleic to ensure shelf stability despite being from high oleic cultivars. This study describes how the concentrations of oleic and linoleic acid changed with maturation of the peanut seeds and affects the purity of individual lots of high and normal oleic types of peanuts. This effect of maturity was seen to be greater in the large seeded Virginia cultivars compared to the smaller seeded market types. Keywords: peanut maturity, shelf-life, lipid stability, seed development 1. Introduction The four peanut market types are Runner, Virginia, Spanish and Valencia. Different peanut products are produced from each cultivar (National Peanut Board, 2020). Runner peanuts, 38 to 70 seeds per ounce, depending on the grade, are used almost entirely for the manufacture of peanut butter but are often used in certain candies. Virginia type peanuts are usually the largest in physical size, 21 to 42 seeds per ounce, and are most often used for snack peanuts sold as “in-shells” or oil roasted peanuts sold in cans or single serve cellophane bags. The Spanish-type, 60 to 80 seeds per ounce, are sold as “redskin” peanuts in cans and may al so be used in confections as they have the highest levels of sweet flavor. Valencia peanuts, 75 to 80 seeds per ounce, represent only about 1% of the USA peanut market and are usually consumed as “in-shell” product although they are sometimes used to produce peanut butter or confections. Peanut seed development takes place entirely underground so that there is no practical way to monitor that development. Like other legumes, peanuts are the seed of the plant and develop from the stem of the flower which forms a peg and moves underground. In the early stages, starch is accumulated to serve as the energy source but then the production of lipid rapidly overtakes it (Pickett, 1950). Initially, the fatty acids produced by the seed are mostly linoleic and palmitic with slightly lesser amounts of oleic acid. As the seed matures, genes coding for fatty acid desaturases are turned on producing enzymes that begin converting oleic acid to linoleic acid. In 1980’s, a mutation was discovered that resulted in peanuts having large amounts of oleic acid compared