197 © Springer International Publishing AG 2018 P. Restani (ed.), Food Supplements Containing Botanicals: Benefts, Side Effects and Regulatory Aspects, DOI 10.1007/978-3-319-62229-3_7 Chapter 7 Protocols for Developing and Testing Methods Applied to the Quality Control of Botanicals Mihaela Badea, Concetta Boniglia, Brunella Carratù, Remigius Chizzola, Chlodwig Franz, Stefania Giammarioli, Maurizio Mosca, Johannes Novak, Paul A. Steenkamp, and Patrizia Restani Abstract Both the application of offcial/published methods and the development of a novel analytical protocol need specifc guidelines for their validation. Methods developed for quality controls of botanicals could be classifed as: (1) phytochemical assays, and DNA-based identifcation; (2) methods for the identifcation and quan- tifcation of specifc compounds. The validation of the methods for phytochemical M. Badea (*) “Transilvania” University of Brasov, UTBV, Brasov, Romania e-mail: badeamihaela1973@gmail.com C. Boniglia • B. Carratù • S. Giammarioli • M. Mosca Department of Food Safety, Nutrition, and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy e-mail: concetta.boniglia@iss.it; brunella.carratu@iss.it; stefania.giammarioli@iss.it; maurizio.mosca@iss.it R. Chizzola Institute for Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Vienna, Austria e-mail: remigius.chizzola@vetmeduni.ac.at J. Novak Institute for Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Vienna, Austria VUW-Bot, Vienna, Austria e-mail: Johannes.Novak@vetmeduni.ac.at C. Franz University of Veterinary Medicine, Vienna, Austria e-mail: chlodwig.franz@vetmeduni.ac.at P.A. Steenkamp CSIR Biosciences, Pretoria, South Africa e-mail: PSteenkamp@csir.co.za P. Restani Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy e-mail: patrizia.restani@unimi.it