776 https://oamjms.eu/index.php/mjms/index Scientifc Foundation SPIROSKI, Skopje, Republic of Macedonia Open Access Macedonian Journal of Medical Sciences. 2021 Sep 04; 9(A):776-781. https://doi.org/10.3889/oamjms.2021.6662 eISSN: 1857-9655 Category: A - Basic Sciences Section: Pharmacology Incision Wound Healing Test of Ethanolic Extract Gel from Salaon (Parsonsia albofavescens [Dennst.] Mabb.) Leaves in Male Rats Nilsya Zebua* , Muharni Saputri, Winda Giovana Sijabat, Inda Aristika Sri Retno Wulandari, Ira Nofriani, Winda Agustiani Zai, Ramadani Arda Arista, Muhammad Suhaimi, Aula Firsa Department of  Pharmacy, Faculty of Pharmacy, Tjut Nyak Dhien University, Sumatera Utara, Indonesia Abstract BACKGROUND: An incision wound is a wound caused by a sharp object. One of the plants that can be used as a traditional medicine for an incision wound is salaon leaves. The prepared form chosen is the gel because it is easy to use and its distribution is faster on the skin. AIM: The aim of the study was to explore whether salaon leaf extract gel meets the quality evaluation requirements and to know the efectiveness of ethanol extract gel of salaon (Parsonsia albofavescens [Dennst] Mabb.) leaves to cure a scar on male rats. METHODS: Experimental method with salaon leaves as sample. Simplicia of salaon leaves extracted by maceration method, and then arenga gum gel-based formulation, afterward evaluation, and testing of its efectiveness on the healing of incision wound of male rats. Normal data were analyzed by the One Way Analysis of variance (ANOVA) and Post Hoc Tuckey Method. Kruskal-Wallis test used for abnormal data. RESULTS: The whole formula shows homogenous results, normal pH, stability, dissemination and good viscosity, less irritating, and with good adhesion. The results of a statistical analysis of the wound on the 8 th day with ANOVA test indicate a signifcant diference between the fve groups p = 0.000 (p < 0.05) and Post-Hoc Tukey Honest Signifcant Diference test results obtained a concentration of 5% not signifcantly diferent from the positive control Placenta extract gel. The optimum dose that can heal incision wound in male rats is 5%. CONCLUSION: The whole formulation qualifes for the quality of the gel formulation. Ethanol extract gel of salaon leaves can heal wound on male rats and the optimal dose is 5%. Edited by: Sinisa Stojanoski Citation: Zebua N, Saputri M, Sijabat WG, Wulandari IA, Nofriani I, Zai WA, Arista RD, Suhaimi M, Firsa A. Incision Wound Healing Test of Ethanolic Extract Gel from Salaon (Parsonsia albofavescens [Dennst.] Mabb.) Leaves in Male Rats. Open Access Maced J Med Sci. 2021 Sep 04; 9(A):776-781. https://doi.org/10.3889/oamjms.2021.6662 Keywords: Arenga gum; Gel; Incision wound; Rats; Salaon leaves *Correspondence: Nilsya Zebua, Tjut Nyak Dhien University, Sumatera Utara, Indonesia. E-mail: nilsya. zebua@gmail.com Received: 18-Jun-2021 Revised: 18-Jul-2021 Accepted: 24-Aug-2021 Copyright: © 2021 Nilsya Zebua, Muharni Saputri, Winda Giovana Sijabat, Inda Aristika Sri Retno Wulandari, Ira Nofriani, Winda Agustiani Zai, Ramadani Arda Arista, Muhammad Suhaimi, Aula Firsa Funding: This research did not receive any fnancial support Competing Interests: The authors have declared that no competing interests exist Open Access: This is an open-access article distributed under the terms of the Creative Commons Attribution- NonCommercial 4.0 International License (CC BY-NC 4.0) Introduction A wound is an end result of a bodily harm that reasons harm and penetrates into the layers of the skin [1]. The basic principle of top-rated wound healing is to minimize tissue damage and provide good enough tissue perfusion and oxygenation, the right nutrients, and a moist wound healing environment to restore the anatomical continuity and characteristic of the afected part [2]. Research on natural ingredients on substances that may accelerate wound healing is one among the items that are presently developing and is being tried by researchers and ancient practitioners around the world. The use of treatments with natural ingredients is becoming increasingly popular today because it has fewer side efects than conventional preparations [3]. The time course of healing for an acute wound usually ranges from 5 to 10 days, or within 30 days [4] One of the plants that can be used as traditional medicine is salaon (Parsonsia albofavescens [Dennst.] Mabb.) leaves. Salaon is derived from the Apocynaceae family. Empirically salaon leaves have been used to treat bruises, gastric wounds, as well as open wounds. In conventional medication inside the Asia Pacifc region, the species Apocynaceae is used to deal with fever, malaria, pain, diabetes, and gastrointestinal diseases [5]. In general, the preparation of wound drugs in the form of liquid or semi-solid form. Half-solid dosage forms such as ointments, creams, and gels are options for all the better ones including longer drug contact and protecting wounds from extraordinary diagnostic. The dosage form of the gel is easier to use and the spread on the skin is faster. In addition, the gel has cooling, moisturizing properties, and easily penetrates the skin to provide a healing efect. Gel preparations can protect the skin from excessive dehydration. The formulation and selection of the right base on the manufacture of gels will afect the number and speed of the active substance to be absorbed. Ideally, the base and carrier should be easy to wear on the skin, not irritating, and safe to use on [6]. In this study, the base of the gel used is gum derived from Arenga pinnata. Gum has been widely used as a thickener, emulsion stabilizer as well as additive in various food and medicine industries. Gum is also known to have antimicrobial and antioxidant properties [7].