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International Journal of Mosquito Research 2014; 1 (2): 28-34
ISSN: 2348-5906
CODEN: IJMRK2
IJMR 2014; 1 (2): 28-34
© 2014 IJMR
Received: 23-03-2014
Accepted: 25-04-2014
Albert O. Mala
(a)Human Health Division, International
Centre of Insect Physiology and Ecology,
P.O. Box 30772-00100, Nairobi, Kenya.
(b)College of Health Sciences, Jomo
Kenyatta University of Agriculture and
Technology, Nairobi, Kenya.
P.O. Box 62000-00100, Nairobi, Kenya.
Lucy W. Irungu
College of Biological and Physical Sciences,
School of Biological Sciences,
University of Nairobi, Kenya.
Email: lirungu@uonbi.ac.ke
Elizabeth K. Mitaki
College of Health Sciences, Jomo
Kenyatta University of Agriculture and
Technology, Nairobi, Kenya.
P.O. Box 62000-00100, Nairobi, Kenya.
Email: mitakielizabeth@gmail.com
Josephat I. Shililu
Human Health Division, International
Centre of Insect Physiology and Ecology,
P.O. Box 30772-00100, Nairobi, Kenya
Email: jshililu@yahoo.com
Charles M. Mbogo
Centre for Geographic Medicine Research
Coast,
Kenya Medical Research Institute,
P.O. Box 230 Kilifi 80108, Kenya
Email: cmbogo@kemri-wellcome.org
Joseph K. Njagi
Malaria Control Unit (MCU)
Ministry of Health
P.O. Box 20750, Nairobi
Email: knjagi@domc.or.ke
John I. Githure
Human Health Division, International
Centre of Insect Physiology and Ecology, P.O.
Box 30772-00100, Nairobi, Kenya.
Email: jgithure@icipe.org.
For Correspondence:
Albert O. Mala
a)Human Health Division,
International, Centre of Insect
Physiology and Ecology,
P.O. Box 30772-00100, Nairobi,
Kenya.
(b)College of Health Sciences, Jomo
Kenyatta University of Agriculture
and Technology, Nairobi, Kenya.
P.O. Box 62000-00100, Nairobi,
Kenya. Email: amala@jkuat.ac.ke
Gonotrophic cycle duration, fecundity and parity
of Anopheles gambiae complex mosquitoes during
an extended period of dry weather in a semi arid
area in Baringo County, Kenya
Albert O. Mala, Lucy W. Irungu, Elizabeth K. Mitaki, Josephat I. Shililu, Charles M.
Mbogo, Joseph K. Njagi, John I. Githure
ABSTRACT
An entomological longitudinal survey was carried out over a 22 month period in two semi-arid villages in
Baringo District in Kenya to study how adult malaria vectors survive under semi-arid conditions and
during extended periods of dry weather.
Methods: Wild caught female mosquitoes were dissected to examine ovarian lobes for parity status and
to determine number of gonotrophic cycles they had undergone. Duration of the first and second
gonotrophic cycles were estimated using cage-reared F1 females. Blood-fed females were kept
individually in plastic vials and percent oviposition incidence recorded.
Results: Significantly fewer mosquitoes laid eggs during the dry than the wet season. The average
duration of the first gonotrophic cycle in the wet season was 4.1 d after blood feeding, 1.1 d (36%) longer
than the dry season (3.0 d). The average duration of the second gonotrophic cycle in the wet season was
2.9 d after second blood meal, 0.7 d (31.8%) longer than those in the dry season. Chi-square tests showed
the gonotrophic cycle duration was significantly shorter during the dry than the wet season. Both
gonotrophic cycle duration and physiological age varied significantly between wet and dry seasons.
Conclusion: These findings suggest the duration of gonotrophic cycle among Anopheles gambiae in dry
lands with scarce breeding sites is shorter during the dry than wet season. Low fecundity rates during the
dry season could be a sign of reduced reproductive activity. However lack of variation in seasonal mating
frequency is a clear indication that oviposition and mating kinetics are influenced differently even under
the same environmental conditions. It is likely that the results of this study will shed an understanding on
spatial and temporal heterogeneities experienced in malaria transmission in semi-arid regions of the
world where malaria and indeed mosquito-borne diseases are a public health menace.
Keywords: Anopheles gambiae, Gonotrophic cycle duration, fecundity, mating behavior.
1. Introduction
Semi-arid areas experience deficits of rain accompanied by high temperatures during dry
seasons and extended periods of dry weather. It is observed that the abundance of the malaria
vector species drops dramatically with the onset of the dry season, and this may depress the
incidence of severe malaria
[1, 2]
. The onset of the rains, however, brings a rapid explosion in
mosquito numbers and a concomitant increase in malaria
[3, 4]
.
Data on the Anophelinae are not conclusive with respect to how each life stage contributes to
long-term survival in semi-arid conditions and the rapid population rise that occurs following
the onset of the rains. Contributions to long-term survival during the dry season and in
semiarid zones could be made during the egg, larval, and/or adult (including pupal) stages.
Adult stages make an important contribution to dry season/ semi-arid population dynamics.
Small changes in the temperature of malaria vector resting sites in the relatively hot semi-arid
areas may have a significant impact on the kinetics of mosquito blood digestion and
consequently in their overall breeding ecology
[5]
. Subsequently, effects may be seen on
population reproduction rates through changes in the duration of the gonotrophic cycle.
Mosquito vectors require blood meals for egg development, and the rate of digestion of these
blood meals is normally directly proportional to increase in temperature.