$VLDQ 3DFLﾀF -RXUQDO RI &DQFHU 3UHYHQWLRQ 9RO   4989 DOI:http://dx.doi.org/10.7314/APJCP.2014.15.12.4989 COX-2 and MMP-2 Gene SNPs and Colorectal Cancer Susceptibility in the Saudi Population Asian Pac J Cancer Prev, 15 (12), 4989-4994 Introduction Colorectal cancer (CRC) is one of the major causes of mortality worldwide. It is ranked the third most common form of cancer worldwide in terms of incidence %URZQ DQG 'X%RLV  ZLWK XQDQLPRXV ﾀQGLQJV WKDW SURVWDJODQGLQV DUH LPSRUWDQW IRU ERWK FDUFLQRJHQHVLV DQG SURJUHVVLRQ *UHHQKRXJK HW DO  *XVWDIVVRQ HW DO D E Clinical and experimental studies UHSRUWHG WKDW &\FORR[\JHQDVH LQKLELWLRQ DWWHQXDWHV DSSHDUDQFH RI HSLWKHOLDO FDQFHU 5XXG HW DO  :KLOH LQGXFHG SURVWDJODQGLQ SURGXFWLRQ SDUWLFXODUO\ 3*( LV NQRZQ WR EH LQYROYHG LQ FHOO VLJQDOLQJ WKURXJK SURVWDQRLG UHFHSWRUV RWKHU REVHUYDWLRQV VXJJHVWHG VXEW\SH (3 UHFHSWRU H[SUHVVLRQ LQ FRORQ FDQFHU WLVVXH WR SUHGLFW UHGXFHG VXUYLYDO *XVWDIVVRQ HW DO D 1 *HQHWLF (QJLQHHULQJ DQG %LRWHFKQRORJ\ 5HVHDUFK ,QVWLWXWH *(%5, &LW\ IRU 6FLHQWLﾀF 5HVHDUFK DQG 7HFKQRORJ\ $SSOLFDWLRQV 2 'HSDUWPHQW RI 0HGLFDO %LRFKHPLVWU\ )DFXOW\ RI 0HGLFLQH 5 ,QVWLWXWH RI *UDGXDWH 6WXGLHV DQG 5HVHDUFK 'HSDUWPHQW RI %LRVFLHQFH DQG 7HFKQRORJ\ $OH[DQGULD 8QLYHUVLW\ $OH[DQGULD (J\SW 3 'HSDUWPHQW RI %LRFKHPLVWU\ &ROOHJH RI 6FLHQFH 4 'HSDUWPHQW RI ,QWHUQDO 0HGLFLQH 'LYLVLRQ RI *DVWURHQWHURORJ\ .LQJ .KDOLG 8QLYHUVLW\ +RVSLWDO .LQJ 6DXG 8QLYHUVLW\ 5L\DGK .6$ )RU FRUUHVSRQGHQFH KHVKDP#KRWPDLOFRP KVDHHG#NVXHGXVD Abstract Background: It has been reported that COX-2 expression is associated with MMP-2 expression in thyroid and breast cancers, suggesting that MMPs are linked to COX-2-mediated carcinogenesis. Several polymorphisms within the MMP2 promoter region have been reported in cases with oncogenesis and tumor progression, especially in colorectal carcinogenesis. Materials and Methods: This research evaluated risk of association of the SNPs, including genes for COX-2 (A/G transition at +202) and MMP-2 (C/T transition at-1306), with colorectal cancer in 125 patients and 125 healthy controls. Results and Conclusions: 2XU GDWD FRQﾀUPHG WKDW MMP2 C-1306 7 PXWDWLRQV ZHUH VLJQLﾀFDQWO\ PRUH FRPPRQ LQ FRORQ FDQFHU SDWLHQWV WKDQ LQ RXU FRQWURO 6DXGL SRSXODWLRQ S 2Q WKH RWKHU KDQG LQ RXU VWXG\ WKHUH ZDV QR VLJQLﾀFDQW DVVRFLDWLRQ EHWZHHQ JHQRW\SH GLVWULEXWLRQ of the COX2 SRO\PRUSKLVP DQG FRORUHFWDO FDQFHU S $Q HOHYDWHG IUHTXHQF\ RI WKH PXWDWHG JHQRW\SH LQ the control group as compared to the patients subjects indeed suggested that this polymorphism could decrease ULVN LQ WKH 6DXGL SRSXODWLRQ 2XU VWXG\ FRQﾀUPHG WKDW WKH SRO\PRUSKLVPV WKDW FRXOG DIIHFW WKH H[SUHVVLRQV RI 003 DQG &2; WKH FRORQ FDQFHU SDWLHQWV ZHUH VLJQLﾀFDQWO\ KLJKHU WKDQ WKDW LQ WKH &2; QHJDWLYH JURXS 7KH IUHTXHQF\ RI LQGLYLGXDOV ZLWK MMP2 polymorphisms in colon cancer patients was higher than individuals with combination of COX2 and MMP2 SRO\PRUSKLVPV 2XU VWXG\ FRQﾀUPHG WKDW LQGLYLGXDOV ZKR FDUULHG WKH polymorphisms that could affect the expressions of COX2 are more susceptible to colon cancer. MMP2 regulatory SRO\PRUSKLVPV FRXOG EH FRQVLGHUHG DV SURWHFWLYH IXUWKHU VWXGLHV QHHG WR FRQﾀUP WKH UHVXOWV ZLWK PRUH VDPSOHV and healthy subjects. Keywords: &RORUHFWDO FDQFHU  VLQJOH QXFOHRWLGH SRO\PRUSKLVP  PDWUL[ PHWDOORSURWHLQDVH  F\FORR[\JHQDVH 5(6($5&+ $57,&/( Associations between Single Nucleotide Polymorphisms of COX-2 and MMP-2 Genes and Colorectal Cancer Susceptibility in the Saudi Population Manal Ali Shalaby 1 , Howaida Attia Nounou 2 , Mohammad Saud Alanazi 3 , Othman Alharby 4 , Nahla Azzam 4 , Hesham Mahmoud Saeed 5 * *HQH H[SUHVVLRQ VWXGLHV LQ FRORQ FDQFHU VKRZHG WKDW WKH DSSHDUDQFH DQG SURJUHVVLRQ RI FRORUHFWDO FDQFHU VHHPV WR EH UHODWHG WR LQFUHDVHG SURGXFWLRQ RI 3*( VHFRQGDU\ to COX-2 LQGXFWLRQ +XOO HW DO  *XVWDIVVRQ HW DO D &DKOLQ HW DO  5XXG HW DO  ,Q DQ RULJLQDO REVHUYDWLRQDO VWXG\ &KDQ HW DO  REVHUYHG WKDW DVSLULQｷV SUHYHQWLYH UROH ZDV HIIHFWLYH RQO\ LQ WKH VXEJURXS RI FRORQ FDQFHUV RYHUH[SUHVVLQJ COX-2 D PHPEHU RI WKH FR[ IDPLO\ RI HQ]\PHV LQ D GRVH DQG WUHDWPHQW GXUDWLRQ GHSHQGHQW PDQQHU 7KH &2; IDPLO\ FRQVLVWV RI WZR LVR]\PHV &2;DQG COX-2. While &2; LV FRQVWLWXWLYHO\ H[SUHVVHG LQ D ZLGH UDQJH RI RUJDQV DQG UHVSRQVLEOH IRU WLVVXH KRPHRVWDVLV COX-2 is LQGXFHG XQGHU LQﾁDPPDWRU\ DQG WXPRU SURPRWLRQ VWLPXOL EHLQJ RYHUH[SUHVVHG LQ DSSUR[LPDWHO\  RI DGHQRPDV DQG  RI FRORQ DGHQRFDUFLQRPDV (EHUKDUW HW DO