Molecular & Biochemical Parasitology 160 (2008) 32–41 Molecular cloning and characterization of Plasmodium falciparum transketolase Shweta Joshi a , Alok Ranjan Singh a , Ashutosh Kumar b , Prakash Chandra Misra c , Mohammad Imran Siddiqi b and Jitendra Kumar Saxena a, * a Division of Biochemistry, Central Drug Research Institute, Lucknow-226001 India b Division of Molecular and Structural Biology, Central Drug Research Institute, Lucknow- 226001, India c Department of Biochemistry, Lucknow University, Lucknow-226001, India Abstract The pentose phosphate pathway (PPP) is an important metabolic pathway for yielding reducing power in the form of NADPH and production of pentose sugar needed for nucleic acid synthesis. Transketolase, the key enzyme of nonoxidative arm of PPP, plays a vital role in the survival/replication of the malarial parasite. This enzyme in Plasmodium falciparum is a novel drug target as it has least homology with the human host. In the present study, the P. falciparum transketolase (PfTk) was expressed, localized and biochemically characterized. The recombinant PfTk harboring transketolase activity catalyzed the oxidation of donor substrates, fructose-6-phosphate (F6P) and hydroxypyruvate (HP), with K m app values of 2.25 and 4.78 mM, respectively. p-Hydroxyphenylpyruvate was a potent inhibitor of PfTk, when hydroxypyruvate was used as a substrate, exhibiting a K i value of 305 µM. At the same time, noncompetitive inhibition was observed with F6P. The native PfTk is a hexamer with subunit molecular weight of 70 kDa, which on treatment with low concentrations of guanidine hydrochloride (GdmCl) dissociated into functionally active dimers. This protein was localized in the cytosol and nucleus of the parasite as studied by confocal microscopy. A model structure of PfTk was constructed based on the crystal structure of the transketolases of S. cerevisae, L. mexicana and E. coli to assess the structural homology. Consistent with the homology modeling predictions, CD analysis indicated that PfTk is composed of 39% α- helices and 26% β-sheets. The availability of a structural model of PfTk and the observed differences in its kinetic properties compared to the host enzyme may facilitate designing of novel inhibitors of PfTk with potential anti-malarial activity. Keywords: Plasmodium falciparum; Transketolase; Pentose phosphate pathway; Hexamer protein Abbreviations: PfTk, P. falciparum transketolase; SEC, size exclusion chromatography; GdmCl, guanidine hydrochloride; pCMB, p-chloromercuribenzoate; NEM, N-ethylmaleimide; EDTA, ethylenediaminetetraacetic acid; HPP, p-hydroxyphenylpyruvate *Corresponding author. Tel.: +91-522-2625932; fax: +91-522-2623405. E-mail address: jkscdri@yahoo.com (J. K. Saxena).