Hind Hadi and Wasan Al Uzri , Int. J. Res. Pharm. Sci., 2020,11(3), 3972-3980 ORIGINAL ARTICLE INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACEUTICAL SCIENCES Published by JK Welfare & Pharmascope Foundation Journal Home Page: www.pharmascope.org/ijrps Cloud point extraction method for the sensitive determination of metoclopramide hydrochloride in pharmaceutical dosage forms Wasan A. Al-Uzri, Hind Hadi * Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq Article History: Received on: 05 Jan 2020 Revised on: 11 feb 2020 Accepted on: 05 Mar 2020 Keywords: Cloud point extraction, Triton X-114, metoclopramide, p-coumaric acid, diazotization coupling reaction ABSTRACT In this work, a simple and very sensitive cloud point extraction (CPE) pro- cess was developed for the determination of trace amount of metoclopramide hydrochloride (MTH) in pharmaceutical dosage forms. The method is based on the extraction of the azo-dye results from the coupling reaction of diazo- tized MTH with p-coumaric acid (p-CA) using nonionic surfactant (Triton X- 114). The extracted azo-dye in the surfactant rich phase was dissolved in ethanol and detected spectrophotometrically at λ max 480 nm. The reaction was studied using both batch and CPE methods (with and without extraction) and a simple comparison between the two methods was performed. The con- ditions that may be affected by the extraction process and the sensitivity of methods were carefully studied. Using optimal conditions, the linearity of calibration curves was in the range of 0.4-13 and 0.05-4 μg/mL and limits of detection of 0.044 and 0.028 μg/mL of MTH for batch and CPE methods respectively. Average recoveries for samples were detected to be between 97- 101 %for both methods, with the relative standard deviation (RSD %) best than 2.7 % and 4.5 % for both methods, respectively. The suggested meth- ods were applied successfully for assay of MTH in commercial pharmaceutical tablets. * Corresponding Author Name: Hind Hadi Phone: Email: hindhadi13@yahoo.com ISSN: 0975-7538 DOI: https://doi.org/10.26452/ijrps.v11i3.2589 Production and Hosted by Pharmascope.org © 2020 | All rights reserved. INTRODUCTION Metoclopramide hydrochloride (MTH), chemically named, 4-amino-5-chloro-N-(2-diethylaminoethyl)- 2-methoxybenzamide monohydrochloride monohy- drate, is a dopamine-receptor antagonist (British Pharmacopoeia, 2001). MTH is usually used as an anti-emetic and in treatment of diabetic gastric sta- sis, which causes symptoms, for instance, nausea, heartburn, decreased appetite, vomiting, and pro- longed fullness after eating. It is also used for its prokinetic and antiemetic properties in disorders of decreased gastrointestinal motility (Mycek et al., 2000). The literature reported different methods for determination of MTH using different techniques involving spectroϑluorimetry (Attia and Aboaly, 2010), spectrophotometry (Bilici et al., 2020), colorimetry using oxygenated graphene quantum dots (Seϑidan and Eskandari, 2019) , LC-ESI-MS (Yan et al., 2010), Flow injection spectrometry (Jia et al., 2010; Al-Arfaj, 2004), ultra-performance liq- uid chromatography (Sowjanya et al., 2013), voltam- metry ((Farghaly et al., 2005), and potentiome- try (Faridbod et al., 2009). Most of the previously reported methods are sensitive but required expen- sive instrumental set up and time-consuming. In contrast, the spectrophotometric technique is still the technique of choice due to its cost-effectiveness and simplicity. MTH was determined using sev- eral spectrophotometric methods based on differ- 3972 © International Journal of Research in Pharmaceutical Sciences