Plant Archives Volume 20 No. 2, 2020 pp. 9714-9720 e-ISSN:2581-6063 (online), ISSN:0972-5210 IDENTIFICATION OF BIOACTIVE INGREDIENTS IN SONCHUS OLERACEUS BY HPLC AND GC/MS Rasha M.M. Mohasib 1 , A. Nagib 2 , A. Abdel Samad 2 , Zeinab A. Salama 1 , Alaa A. Gaafar 1 , Hanan A.A. Taie 1 and Sameh R. Hussein 3 1 Plant Biochemistry Department, National Research Centre, Dokki, Giza, Egypt. 2 Biochemistry Department, Faculty of Agricultural, Cairo university, Dokki, Giza, Egypt. 3 Department of Phytochemistry and Plant Systematics, National Research Centre, 33 El Bohouth St., Dokki, Giza, Egypt. Abstract This study aims to evaluate the successive extraction of the active ingredients and their anticancer activity of arial parts (stem, leaves and flowers) of S. oleaceous. Therefore, Sonchus oleraceus aerial parts were subjected to a successive extraction by using solvent of different polarities; each fraction was evaluated for its anticancer. Each active extract was farther biogided fractionated to determine and identify the active compound/s present in each sub-fraction. All the tested species were extracted using four solvents: hexane, ethyl acetate, methanol and water. Phenols, flavonoids and tannins were quantified for all extracts. The chemical analysis proved it to will be a good source of protein, fat and carbohydrates. The results showed that the percentages of moisture content (92.72%), ash content (15.14%), crude protein (25.94%), crude lipid (4.05%) and carbohydrate (54.87%) respectively of the S. oleraceus L. the highest content of total phenolic (TP), total flavonoid (TF) and total tannin (TT) was obtained by methanol fraction. The active ingredients were evaluated as well employing gas chromatography coupled to mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). Gas chromatography mass spectrometry (GC-MS) data showed the identification of ten volatile compounds in hexane fraction of the Sonchus oleraceus, among them two were found to be the major components 9 Octadecenamide (CAS) 40.92% and 1 Hexacosanol 21.01%. The HPLC analysis of phenolic compounds confirmed that the water fraction of Sonchus oleraceus L detected high amounts of pyrogallol and chlorogenic respectively. The result showed that pronounced cytotoxic activity of cancer cells at 100 µg/ml in aqueous fraction in breast cancer (94.8%), while in Methanol extract in Colon Carcinoma (88%) On the other hand, ethyl acetate fraction of the Sonchus Oleraceus showed the highest percentage of colon cancer cells (82.6%) and breast cancer (80%), while the ethyl acetate extract in hepatocellular carcinoma cell line showed moderate effect (46.3%). Thus, our results highlight of Sonchus oleraceus L for its possible clinical use to oppose malignancy development against mostly breast and colon cell line as a bio agent in pharmaceutical industries. Key words: Sonchus oleraceus; HPLC; GC/MS; anti-proliferative. Introduction Herbs are still used by 80% of world population, because of their easy availability and very negligible side effects. From ancient period medicinal plants are used for pharmacological studies. The Asteraceae (commonly known as sunflower) is a large and widespread family which contain many genera. Sonchus oleraceus is a plant belongs to family asteraceae (Hussain et al., 2010). The Sonchus oleraceus plant, popularly known as milkweed, milkweed-smooth is a cosmopolitan species, found in many agricultural regions like weed crops. The S. oleraceus is native to Europe, North Africa and West Asia. It has spread to North and South America, India, China, southern Australia (Cameron, 2000). The genus Sonchus comprises about 60 species and three of them have become common weeds around the world. Sonchus contains variety of phytochemical compounds such as sesquiterpene lactones of the eudismanolide and guaianolide structures (ElkHayat, 2009) also contain flavonoids, flavanols, proanthocyanins, total phenol, saponins, phytate and alkaloids (Jimoh et al., 2011). High concentration of fatty acids, vitamin C, carotenoids, oxalic acid and high mineral contents which gave this plant high