Journal of Pharmacology & Clinical Research ISSN: 2473-5574 Research Article Volume 4 Issue 5 - December 2017 DOI: 10.19080/JPCR.2017.04.555646 J of Pharmacol & Clin Res Copyright © All rights are reserved by Ashish Sarkar Effects on Plant Metabolites of Bauhinia Purpurea Linn Ashish Sarkar* 1 , LP Singh 1 , VD Tripathi 2 and Garima Mishra 3 1Institute of Pharmacy, HCPG College, India 2Sagar Institute of Technology, India 3Department of Pharmaceutical science, Shri Guru Ram Rai University, India Submission: December 12, 2017; Published: December 19, 2017 *Corresponding author: Ashish Sarkar, Institute of Pharmacy, HCPG College, Varanasi, Uttar Pradesh, India, Tel: ; Email: Introduction The plant is popular in India, bark was reported as anti mycobacterial, antimalarial, antifungal, cytotoxic and anti- inflammatory activities. The leaves were reported to possess antinociceptive, anti inflammatory and antipyretic properties, while the stem was found to have anti-diabetic and adrenergic properties, Bauhinia statins, isolated from leaves and bark was reported to inhibit human cancer cell [5-8]. Bauhinia Purpurea linn widely distributed in Himalayan region of India [9-13]. Traditionally Bauhinia Purpurea linn are intended to use for the treatment of numerous activity namely diarrhea, ulcers, enlarge cervical glands, goiter, scrofulous tumors etc. Bauhinia Purpurea linn extracts was scientifically documented for its antinociceptive, antidiarrhoeal, anti-inflammatory, analgesic, anticancer, antipyretic, antimalarial, antimycobacterial, antifungal, anti-diabetics and anti-diarrheal activity etc [14-17]. Inflammatory and arthritis activity of ethanolic extract of Bauhinia Purpurea bark was previously reported. Bauhinia Purpurea linn contains various types of phytochemicals like polyphenols and flavanoids which area responsible for anti- inflammatory and antiarthritis activity. The constituents of a various medicinal plants may fluctuate while changing in atmosphere and time of collection. Because the plant extracts are used all seasons, it was intended to study changes of chemical constituents if any found while changing season and reason. The samples for this study were collected in summer, monsoon and winter during 2013. Similarly for observing changes in geographical conditions plants samples are collected from Madhya Pradesh, and Himachal Pradesh [18-20]. Materials and Methods For present study the fresh leaves of Bauhinia Purpurea linn was selected. The plant was authenticated by Dr. NK Dubey, Department of Botany, Banaras Hindu University, and Varanasi (U.P.). The dried leaves were reduced to powder and stored moisture free environment till use. Preparation of Extracts Approximately 1 Kg of Bauhinia Purpurea linn leaves of the plant were packed in soxhlet apparatus and successively extracted with petroleum ether, hydroalcohol and water separately, till the completion of the extraction. The extract was filtered on hot condition further distillation is carried out for removal of solvent completely; samples are dried in a desiccator for removal of moisture and further use. Determination of Total Phenol Content Polyphenol content was determined spectrophotometrically. 1.0 ml of prepared extract were reacted with Folin- Ciocalteu reagent for oxidation, during reaction 1.8 ml of sodium Abstract Bauhinia purpurea Linn is an ornamental plant found throughout the globe known as khairwal in Hindi having high therapeutic value in Ayurvedic preparations [1]. Numerous types of biological activities are attributed to bauhinia species. B. Purpurea Linn is an important species used to treat many ailments in traditional system of medicine [2]. The plant is useful in diarrhoea, pain, rheumatism, inflammation, cancer and many more medicinal perpuse [3,4]. Since the plant is used in various formulations commonly, its quality and quantity is a prime importance. Current study deals with the changes of chemical constituents due to different factors. The findings of the study suggested that Hydroalcoholic extracts contains strong phytochemical activity while changing different parameters. Keywords: Bauhinia Purpurea Linn; Hydro Alcoholic; Chemical Constituents; Antioxidant Activity J of Pharmacol & Clin Res 4(5): JPCR.MS.ID. 555646 (2017) 001