Acta Scientific Pharmaceutical Sciences (ISSN: 2581-5423) Volume 3 Issue 6 June 2019 Pharmacological Probing of Two Locally Available Shrubs Namely, Cardiospermum halicacabum (Mudakatthaan) and Mentha spicata (Pudina) Savitha C, Vigneshwari T, Raga Priyanka C, Keerthika S and Shaleesha A Stanley* Department of Biotechnology, Jeppiaar Engineering College, Chennai, Tamil Nadu, India *Corresponding Author: Shaleesha A Stanley, Professor, Department of Biotechnology, Jeppiaar Engineering College, Chennai, Tamil Nadu, India. Research Article Received: March 12, 2019; Published: May 22, 2019 Citation: Shaleesha A Stanley., et al. “Pharmacological Probing of Two Locally Available Shrubs Namely, Cardiospermum halicacabum (Mudakatthaan) and Mentha spicata (Pudina)”. Acta Scientific Pharmaceutical Sciences 3.6 (2019): 153-155. Keywords: Cardiospermum halicacabum, Mentha spicata, Anti Bacterial; Phyto-Chemical; Herbal The study analysed the antibacterial property of fresh and dry leaf extracts obtained from Cardiospermum Halicacabum and Mentha spicata against few bacterial strains that include both gram positive and negative (Escherichia coli, Bacillus subtilis, Pseudomonas aeuroginosa, Brucella, Pseudomonas fluroscens and Bacillus cereus). The fresh and dry leaf extracts were made through different solvents like isopropanol, methanol, chloroform and aqueous extracts. Abstract Introduction Antibiotic resistance is surging severely and novel mechanism of resistance has spread globally and has led to difficulty in treat- ments [1]. The acceptance of herbal medicine for healthcare has made screening of the plants for active compounds vital as they serve as a novel antibiotic source. Cardiospermum Halicacabum, a climber about 2-4 m long locally spelled as Mudakathan keerai plays an important role in traditional medicine and is commonly used for treatment of cough, hyperthermia, arthritis, stiffness of limbs, snake bite, rheumatism [3]. Reports have revealed the presence of anxiolytic, antipyretic, antiarthritic, anti-ulcer, anti- hyperglycaemic activity in C. Halicacabum [2-5]. Mentha spicata, a 30-100 cm long plant commonly known as Mint (English) and Pu- dina (Tamil) hold a high dietary medical repute and used as a do- mestic herbal remedy. M. spicata is diuretic, stomachic, stimulant, antisemetic and used as a traditional treatment for cancer [4,5]. This study is an attempt to probe the pharmacological properties of Cardiospermum halicacabum and Mentha spicata as it is widely consumed as green leafy vegetable in all parts of Tamil Nadu. The extracts were prepared by cold percolation method from the fresh and dry leaves of Cardiospermum halicacabum and Men- tha spicata in different solvents such as ethanol, isopropanol, chlo- roform, methanol and aqueous water. The extracts were screened against few bacterial strains that include both gram positive and negative (Escherichia coli, Bacillus subtilis, Pseudomonas aeurogi- nosa, Brucella, Pseudomonas fluroscens and Bacillus cereus) by disc diffusion method [6]. The samples were subjected to phyto- chemical screening namely, tannin, saponin, flavonoids, steroids, proteins, carbohydrates, alkaloids, terpenoids, anthraquinone and glycoside [7]. The isopropanol extracts of fresh and dry leaves of Cardiospermum halicacabum and Mentha spicata were subjected to spectrometric measurement in the frequency range of 4000-450 cm-1 in FT-IR Perkin Elmer 2000 model (Thermo Fisher Scientific., Inc, USA) for identifying the functional groups. Materials and Methods Presence of phytochemicals and flavonoids were determined by quality color tests and the functional groups of flavonoids and acids were confirmed by FT-IR. The isopropanol extracts of dry leaves in both the plants showed maximum antibacterial inhibition towards gram negative strain and the study confirms that the leaves of Mentha spicata and Cardiospermum halicacabum can be used as herbal remedy and against infectious conditions. DOI: 10.31080/ASPS.2019.03.0290