Research Article Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice Dwi Winarni , 1 Fitria Nikmatul Husna, 1 Muhammad Farraz Syadzha, 1 Raden Joko Kuncoroningrat Susilo , 1 Suhailah Hayaza, 1 Arif Nur Muhammad Ansori , 1,2 Mochammad Amin Alamsjah, 3 Muhamad Nur Ghoyatul Amin, 3 Pugar Arga Christina Wulandari, 4 Pratiwi Pudjiastuti, 4 and Khalijah Awang 5 1 Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia 2 Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia 3 Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya 60115, Indonesia 4 Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia 5 Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia CorrespondenceshouldbeaddressedtoDwiWinarni;dwi-w@fst.unair.ac.id Received 16 June 2021; Revised 20 December 2021; Accepted 13 January 2022; Published 9 February 2022 AcademicEditor:PaolaBrun Copyright©2022DwiWinarnietal.isisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. is research aimed to determine the topical administration effect of the combination of Sargassum duplicatum and Garcinia mangostana extractstoamelioratediabeticopenwoundhealing.estudyused24adultmalesof Mus musculus (BALB/cstrain, 3–4 months, 30–40g). ey were divided into normal control groups (KN) and diabetic groups. e diabetic group was streptozotocin-induced and divided further into three treatment groups: the diabetic control group (KD), the S. duplicatum treatmentgroup(PA),andthecombinationof S. duplicatum and G. mangostana treatmentgroup(PAM).edoseoftreatment was50mg/kgofbodyweight.Eachgroupwasdividedintothreetreatmentdurations,whichwere3days,7days,and14days.e wound healing process was determined by wound width, the number of neutrophils, macrophages, fibroblasts, fibrocytes, and collagen density. Histological observation showed that the topical administration of combination extracts increased the re- epithelializationofthewoundedarea,fibroblasts,fibrocytes,andcollagensynthesis.etopicaladministrationofcombination extractsalsodecreasedthenumberofneutrophilsandmacrophages.isstudyconcludedthatthetopicaladministrationofthe combination of S. duplicatum and G. mangostana extracts improved the open wound healing process in diabetic mice. 1. Introduction Diabetes mellitus (DM) is a group of metabolic disorders characterized by hyperglycemia caused by abnormalities in theprocessofinsulinsecretion,insulinperformance,orboth [1]. ere was a prevalence of about 9.6% of the world’s diabetes population in 2013, and it tends to increase until 9.9%in2035[2].Hyperglycemiaisassociatedwithincreased reactiveoxygenspecies(ROS),whichtriggersanincreasein oxidative stress [3]. Hyperglycemia leads to the accumulation of ROS in tissues or cells with different metabolic pathways, such as activation of the polyol path- way,formationofadvancedglycationendproducts(AGEs) andactivationofitsreceptor,activationofisoformprotein kinase C (PKC), increased activity of hexosamine pathway, and decreased antioxidant defenses. e accumulation of ROS can cause an oxidant-endogenous antioxidant imbal- ance which triggers the activation of sensitive intracellular signaling pathways stress, and this condition triggers cell damage and causes diabetes complications [4]. Hindawi Scientifica Volume 2022, Article ID 9700794, 7 pages https://doi.org/10.1155/2022/9700794