1
Supporting Information
Dual Functional Liposome Specifically Target Melanoma Cells Through
Integrin and Ephrin Receptors
Debmalya Bhunia
#a
, Abhijit Saha
# a
, Anindyasundar Adak
a
, Gaurav Das
b
and Surajit Ghosh
*a,b
a
Organic & Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 4
Raja S. C. Mullick Road, Jadavpur, Kolkata-700032, West Bengal, India. Fax: +91-33-
2473-5197/0284; Tel: +91-33-2499-5872
b
Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of
Chemical Biology Campus, 4 Raja S. C. Mullick Road, Kolkata 700 032, India
Correspondence author: sghosh@iicb.res.in
Experimental Section
Materials:
Chemicals: Wang resin and all required fmoc protected amino acids were purchased from
Novabiochem (Merck). N,N’-dimethylformamide (DMF), dichloromethane (DCM),
trifluoroacetic acid (TFA), pipyridine, O-(benzotriazol-1-yl)-N,N,N′,N′-
tetramethyluroniumhexafluorophosphate (HBTU), diisopropylethylamine (DIPEA), methanol
and di-tert-butyl dicarbonate were purchased from Spectrochem. 1,4-dioxan was purchased
from RANKEM. Ethanedithiol (EDT) and phenol were purchased from Merck. 5,6 carboxy
fluorescence, 5-diphenyltetrazolium bromide (MTT), dulbecco’s modified eagle’s medium
(DMEM), kanamycin sulfate, trypsin-EDTA solution, dimethyl sulfoxide for cell culture and
formaldehyde solution for molecular biology were purchased from Sigma Aldrich. 1-
Bromohexadecane and ethelene diamine were purchased from sigma. Penicillin-Streptomycin
and fetal bovine serum (FBS) were purchased from Invitrogen. Annexin V and propidium
iodide apoptosis detection kit were purchased from Santa Cruz Biotechnology. Bisbenzimide
H 33258 (hoechst) was purchased from Calbiochem. Cholesterol and DOPC were purchased
from Avanti polar lipid. Chloroform was purchased from Merck. Cover glass bottom dishes
were purchased from SPL. Agarose was purchased from Fisher scientific. Goat polyclonal
anti-Rabbit IgG was purchased from Abcam. These compounds were used without further
purification. We used Shimadzu HPLC equipped with C-18 semi preparative reverse phase
column for peptide purification. Experimental water was used of milli Q grade. HPLC grade
acetonitrile was purchased from J.T. Baker. Olympus IX83 inverted microscope was used for
cell imaging. Synthesized lipopeptide masses were characterized by MALDI-TOF.
Preparation of LDV and YSA tripeptide: About 300 mg wang resin was taken in a peptide
vessel and swelled for overnight in DMF-DCM (1:1) solvent. Five equivalent of excess fmoc
protected valine, aspartic acid and leucine were coupled successively followed by fmoc
deprotection by CEM microwave peptide synthesizer equipped with Liberty1. Coupling and
deprotection steps were maintained for eight and five minutes respectively and microwave
power was 35 watt. Five equivalent excess of N,N’-Diisopropylethylamine (DIPEA) and
Electronic Supplementary Material (ESI) for RSC Advances.
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