1 Supporting Information Dual Functional Liposome Specifically Target Melanoma Cells Through Integrin and Ephrin Receptors Debmalya Bhunia #a , Abhijit Saha # a , Anindyasundar Adak a , Gaurav Das b and Surajit Ghosh *a,b a Organic & Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata-700032, West Bengal, India. Fax: +91-33- 2473-5197/0284; Tel: +91-33-2499-5872 b Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Chemical Biology Campus, 4 Raja S. C. Mullick Road, Kolkata 700 032, India Correspondence author: sghosh@iicb.res.in Experimental Section Materials: Chemicals: Wang resin and all required fmoc protected amino acids were purchased from Novabiochem (Merck). N,N’-dimethylformamide (DMF), dichloromethane (DCM), trifluoroacetic acid (TFA), pipyridine, O-(benzotriazol-1-yl)-N,N,N′,N′- tetramethyluroniumhexafluorophosphate (HBTU), diisopropylethylamine (DIPEA), methanol and di-tert-butyl dicarbonate were purchased from Spectrochem. 1,4-dioxan was purchased from RANKEM. Ethanedithiol (EDT) and phenol were purchased from Merck. 5,6 carboxy fluorescence, 5-diphenyltetrazolium bromide (MTT), dulbecco’s modified eagle’s medium (DMEM), kanamycin sulfate, trypsin-EDTA solution, dimethyl sulfoxide for cell culture and formaldehyde solution for molecular biology were purchased from Sigma Aldrich. 1- Bromohexadecane and ethelene diamine were purchased from sigma. Penicillin-Streptomycin and fetal bovine serum (FBS) were purchased from Invitrogen. Annexin V and propidium iodide apoptosis detection kit were purchased from Santa Cruz Biotechnology. Bisbenzimide H 33258 (hoechst) was purchased from Calbiochem. Cholesterol and DOPC were purchased from Avanti polar lipid. Chloroform was purchased from Merck. Cover glass bottom dishes were purchased from SPL. Agarose was purchased from Fisher scientific. Goat polyclonal anti-Rabbit IgG was purchased from Abcam. These compounds were used without further purification. We used Shimadzu HPLC equipped with C-18 semi preparative reverse phase column for peptide purification. Experimental water was used of milli Q grade. HPLC grade acetonitrile was purchased from J.T. Baker. Olympus IX83 inverted microscope was used for cell imaging. Synthesized lipopeptide masses were characterized by MALDI-TOF. Preparation of LDV and YSA tripeptide: About 300 mg wang resin was taken in a peptide vessel and swelled for overnight in DMF-DCM (1:1) solvent. Five equivalent of excess fmoc protected valine, aspartic acid and leucine were coupled successively followed by fmoc deprotection by CEM microwave peptide synthesizer equipped with Liberty1. Coupling and deprotection steps were maintained for eight and five minutes respectively and microwave power was 35 watt. Five equivalent excess of N,N’-Diisopropylethylamine (DIPEA) and Electronic Supplementary Material (ESI) for RSC Advances. This journal is © The Royal Society of Chemistry 2016