~ 1492 ~ International Journal of Chemical Studies 2018; 6(5): 1492-1495 P-ISSN: 2349–8528 E-ISSN: 2321–4902 IJCS 2018; 6(5): 1492-1495 © 2018 IJCS Received: 11-07-2018 Accepted: 15-08-2018 Arunkumar K Kambl Department of Fruit Science, College of Horticulture, UHS, Bagalkot, Karnataka, India Mukunda GK Professor of Horticulture, Department of Horticulture, UAS, Bangalore, Karnataka, India Namita B Raut Department of vegetable science, College of Horticulture, UHS, Bagalkot, Karnataka, India Nachegowda V Professor of Fruit science, Directorate of research, UHS Bagalkot, Karnataka, India Murthy BNS Senior Scientist, Division of fruit science, IIHR Bangalore, Karnataka, India Nagarajaiah Professor of Botany, UAS, Bangalore, Karnataka, India K Seenappa Professor of Statistics, UAS Bangalore, Karnataka, India Correspondence Arunkumar K Kambl Department of Fruit Science, College of Horticulture, UHS, Bagalkot, Karnataka, India Role of bio inoculants on production of primary, secondary and tertiary roots in grapes cuttings ( Vitis vinefera L.) with special reference to wine varieties Arunkumar K Kambl, Mukunda GK, Namita B Raut, Nachegowda V, Murthy BNS, Nagarajaiah and K Seenappa Abstract The experiment was conducted at Division of Horticulture, Gandhi Krishi Vignana Kendra, University of Agricultural Sciences, Bangalore, There were five varieties viz. Arka Kanchan, Arka Soma, Arka Thrishna, Bangalore Blue and Queen of Vine Yard, consisting of ten treatments and the treatments consisting of Trichoderma harzianum, Glomus mossae, PSB and IBA, replicated in five and tested in a factorial completely randomized design. The results revealed that among the treatmetns, maximum number of primary, secondary and tertiary roots was recorded in treatment T8 (30.65, 84.37 and 37.05 respectively). Whereas, lowest number of primary, secondary and tertiary roots was noticed in the treatment T10 (15.34, 56.51 and 25.69 respectively). Among the varieties maximum number of primary and secondary roots was recorded in Bangalore Blue (25.15 and 79.14), Whereas incase of tertiary roots were observed in Arka Thrishna (33.28). However, least number of primary and tertiary roots was noticed in Queen of Vine Yard (19.38 and 27.61), Where as incase of secondary roots were observed in Arka Soma (59.12). Interaction effect between the treatments and varieties maximum number of primary roots was noticed in variety Bangalore Blue with treatment T8 (33.86), Where as lowest number of primary roots were recorded in Queen of Vine Yard with treatment T10 (12.64). Maximum number of Secondary roots was noticed in variety Arka Krishna with treatment T8 (95.84), Where as lowest number of secondary roots were recorded in Arka Soma with treatment T10 (49.52). Maximum number of tertiary roots was noticed in variety Arka Thrishna with treatment T8 (41.56), Where as lowest number of tertiary roots were recorded in Queen of Vine Yard with treatment T10 (23.65). Keywords: grape varieties, biofertilisers, IBA, primary, secondary and tertiary roots Introduction Propagation is one of the most important aspects in horticulture. Vegetative propagation methods like cuttings, air layering, grafting and budding are being widely followed to raise plants of desired genetic constitution and to maintain their purity for commercial exploitation. Grape (Vitis vinifera L.) is one of the most important fruit crop of tropical and sub-tropical regions. It has been cultivated from time immemorial. Globally it occupies an area of 6976108 ha with an annual production of 68412467 MT. In India it has been cultivated in 1187000 ha with an annual production of 2585.3 MT (Anonymous, 2015) [5] . Propagation by stem cuttings is the commercial method followed in grapes root stock.... etc. Further, growth substances applied exogenously to cuttings are found to enhance early and good root formation. Soil microorganisms have been differentiated according to their functions by soil microbiologists and microbial ecologists, as benefial and harmful. Benificial microorganisms are those that can stimulate plant growth by fixing atmospheric nitrogen, decomposing organic wastes and residues, enhance nutrient cycling, detoxifying pesticides, suppressing plant dieses and soil borne pathogens by producing bioactive compounds such as vitamins, hormones and enzymes. Using some of these beneficial microorganisms, various microbial inoculants have been prepared for use in crop propagation and production to reduce the cost on synthetic chemicals and to minimize environmental pollution. Since with use of synthetic chemicals and pesticides, they are now widely applied in eco technology. Microorganisms like Trichoderma spp, Glomus spp and some bacteria are reported to produce phytohormones (Constracerta and Vandelyden, 1995) [2] which induce rooting of different plant species.