Genetic Stability Assessment of in Vitro Rooted Populus Alba L. Micro-Shoots in Diferent Media compositions 1 MedDocs Publishers Received: Dec 10, 2021 Accepted: Feb 15, 2022 Published Online: Feb 18, 2022 Journal: Journal of Plant Biology and Crop Research Publisher: MedDocs Publishers LLC Online editon: htp://meddocsonline.org/ Copyright: © Tawfk E (2022). This Artcle is distributed under the terms of Creatve Commons Atributon 4.0 Internatonal License *Corresponding Author(s): Eman Tawfk Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt. Email: emantawfk@science.helwan.edu.eg Cite this artcle: Tawfk E, Ahmed MF. Genetc Stability Assessment of in Vitro Rooted Populus Alba L. Micro-Shoots in Diferent Media compositons. J Plant Biol Crop Res. 2022; 5(1): 1061. Journal of Plant Biology and Crop Research Open Access | Research Artcle ISSN: 2637-7721 Abstract Populus alba is a huge woody plant. The bark is very white and smooth when the tree is young. With age, the bark be- comes darker and more furrowed. This work manipulate the idea of variaton of the new individual resulted from plant tssue culture due to any change in media compositon. Pre- viously it was known that all the new resulted individuals are genetcally similar, but in this study, the contrast was proved. As the new individuals are genetcally diferent. The plant was multplied in shootng and rootng MS media. This study was designed based on two parameters (media power and hormone concentratons) resultng in 25 treatments. It was known that the new plantlets from tssue culture were identcal, similar to the source mother plant and each other as well. Nevertheless, the current study manipulated the ef- fect of various media power and auxins concentratons on P. alba’s molecular responses. Hence, the genetc stability of the resulted new individuals from treatments is completely diferent. Also the diferent auxin hormone concentraton (IBA and NAA) made genetc instability in the new resulted individuals. A molecular marker (RAPD-PCR) was used to estmate the genetc variatons. Four decamers yielded sor- able bands and led to a total polymorphism percentage of 48.26. The morphological and physiological variaton origi- nated from the genetc variaton these results confrm that the new resulted plantlets from diferent tssue culture con- ditons could be diferent from each other and led to difer- ent growth parameters. Eman Tawfk 1 *; Mohamed Fathy Ahmed 2 1 Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt. 2 Hortculture Department, Faculty of Agriculture, Ain Shams University, Egypt. Keywords: Populus alba; Tissue culture; Micropropagaton; RAPD-PCR; Genetc instability.