Journal of International Dental and Medical Research ISSN 1309-100X HPV genotypes in SCC patients in Yogyakarta http://www.jidmr.com Isadora Gracia and et al Volume ∙ 11 ∙ Number ∙ 3 ∙ 2018 Page 866 Oral Squamous Cell Carcinoma Positive for HPV Genotypes 16 and 18: A Retrospective Clinicopathologic Study in Yogyakarta, Indonesia Isadora Gracia 1,2 *, Irianiwati 3, Totok Utoro 3 , Supriatno 4 , Indwiani Astuti 5 , Didik Setyo Heriyanto 3 , Dibyo Pramono 6 1. Doctoral Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia. 2. Departement of Dental Medicine, School of Medicine and Health Science, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia. 3. Departement of Anatomic Pathology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia. 4. Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia. 5. Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia. 6. Department of Dental Public Health, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia. Abstract Human papillomavirus (HPV) is closely related with the incidence of OSCC. Few studies conducted in Indonesia have focused on the association of HPV genotypes with OSCC. In this study, the prevalence of HPV infection and correlate it with the clinicopathologic features of OSCC patients was investigated. This retrospective study involved 64 FFPE blocks which diagnosed as OSCC in the year of 2011–2015. DNA extraction was conducted with a commercial kit. HPV-positive samples were genotyped using a commercial kit. Bivariate analysis was conducted to correlate clinicopathologic features with HPV genotypes. Thirty-five out of 64 (54.7%) OSCC samples were positive for HPV. Of these cases, eight (22.86%) and 3 (8.57%) cases were positive for HPV genotypes 18 and 16, respectively. HPV infection was found in 23 out of 38 (60.50%) male patients and 22 out of 36 (61.10%) female patients aged ≥55 years old. HPV DNA was detected in 22 out of 37 (59.5%) tongue samples and in 28 out of 49 (57.1%) samples with well-differentiated histopathologic features. Results suggested that 54.7% of OSCC patients were HPV infected. The tongue was the most common anatomical site for HPV-positive OSCC. The prevalence of well-differentiated HPV- positive OSCC was 57.1%. Experimental article (J Int Dent Med Res 2018; 11(3): 866-871) Keywords: OSCC, HPV genotype 16 and 18, Clinicopathologic. Received date: 26 April 2018 Accept date: 27 May 2018 Introduction Oral squamous cell carcinoma (OSCC) is the sixth most commonly reported malignancy in the world and is particularly prevalent in Southeast Asia. 1,2 Human papillomavirus (HPV) has an important role in the carcinogenesis of several malignancies, including OSCC. 3,4 HPV is member of the Papillomaviridae family. It is a non-enveloped, small, circular double-stranded DNA virus approximately 8000 base pairs in length. It infects the epithelial tissues of the epidermis and mucosa. High risk (HR) genotypes are 2.8 times more commonly detected in OSCCs than low risk (LR) genotypes. 5,6 A recent study reported that HR genotypes, especially HPV genotype 16, is an etiological factor of OSCC. 1 Epidemiological studies have revealed the high prevalence of HPV-induced OSCC in Asian countries. Asian countries have the highest HPV prevalence (0-100%) followed by Europe (0-95%), the United States of America (0-70%), and Africa (0-11.9%). 7 Ndiaye et al. (2014) reviewed 72 papers published during the period of 2000-2012 and found that HPV-positive OSCC has a prevalence of 24.2%. 8 Zhu et al. (2012) conducted a meta-analysis of 18 case control studies published during 1994-2011 and found *Corresponding author: Dr. Isadora Gracia Doctoral Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia E-mail: isadora.gracia@gmail.com