INTRODUCTION Storing insecticide including botanical insecticide formulations becomes critical control points in pesticide distribution and pesticide storage. The quality of formulation, safety, and performance should be stable during a storage process. Additionally, pesticide containers have an important efect on storage and shelf life. The stability data of pesticide formulation during storageis required for pesticidal products registration (CIPAC Handbook, 1980). One indicator of pesticide formulation performance is insecticidal activity against targeted pest. Tephrosia vogelii and Piper aduncum mixture formulation in form of emulsifable concentrate (EC) and wettable powder (WP) have a strong insecticidal activity against C. pavonana with LC 50 and LC 95 of EC formulation were 0.15% and 0.13% and WP formulation were 0.35% and 0.31%. Besides causing the mortality of C. pavonana, EC and WP formulations inhibited the development of treatment larvae (Lina, Manuwoto, Syahbirin, & Dadang, 2017). T. vogelii leaves contain isofavonoids compounds known as rotenon, elliptone and other rotenoid compounds namely deguelin and tefrosin (Mkenda & Ndakidemi, 2014; Mkenda et al., 2015). The bioactive compounds from T. vogelii ofer great potential of developing botanical pesticides against post harvest insects in storing (Mkenda & Ndakidemi, 2014). Abizar & Prijono (2010) reported ethyl acetate leaf extracts of T. vogelii toxic to C. pavonana larvae and at LC 95 level a mixture of leaf extract of purple- fowered T. vogelii and fruit extract of P cubeba (5:9, w/w) was more toxic to C. pavonana larvae than each extract tested separately. Dilapiol from P. aduncum can inhibit the activity of cytochrome P450 enzymes in microsomes of gastrointestinal cells of corn borer larvae Ostrinianubilalis. The synergistic character of dilapiol is very benefcial for the development of botanical insecticides as an AGRIVITA Journal of Agricultural Science AGRIVITA Journal of Agricultural Science. 2018. 40(3): 498-505 Storage Temperature of Botanical Insecticide Mixture Formulations and Its Activity Againsts Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) Eka Candra Lina* ) , Nia Yulianti, Gustria Ernis, Arneti and Novri Nelly Department of Plant Protection, Faculty of Agriculture, Andalas University, Kampus Unand Limau Manis, Padang, Indonesia ARTICLE INFO Keywords: Botanical insecticide Crocidolomia pavonana Formulation Storage Temperature Article History: Received: February 16, 2018 Accepted: August 10, 2018 *) Corresponding author: E-mail: ekacandra222@gmail.com, eka_candra@faperta.unand.ac.id ABSTRACT The purpose of this research was to evaluate the safety of mixture formulations of Tephrosia vogelii and Piper aduncum at various storage temperatures and their insecticidal activity against C. pavonana larvae. Formulations were made from T. vogelii and P. aduncum (1:5) in emulsifable concentrate (EC) and wettable powder (WP) form. Both formulations have strong insecticidal activity against C. pavonana. This research used a randomized completed design in diferent temperature treatments. Insecticidal activity of formulations after storage was tested against C. pavonana. The treatments were done using deep leaf method in 5 concentrations and 5 replications. The results showed that LC 95 value of EC formulation after storage at condition: below 4°C, room temperature, and 40°C were 0.19%, 0.34% and 0.21% respectively. Based on LC 95 value of EC formulation from each treatment, indicated that EC formulation after storage could hold insecticidal activity as good as insecticidal activity before storage due to LC 95 value after storage relatively lower than LC 95 value before storage (0.35%). In the contrary, WP formulation activity was signifcantly decreased in all treatments compared to WP formulation activity before storage based on LC 95 value. ISSN: 0126-0537 Accredited by DIKTI Decree No: 60/E/KPT/2016 Cite this as: Lina, E. C., Yulianti, N., Ernis, G., Arneti, & Nelly, N. (2018). Storage temperature of botanical insecticide mixture formulations and its activity againsts Crocidolomia pavonana (F.) (Lepidoptera: Crambidae). AGRIVITA Journal of Agricultural Science, 40(3), 498–505. http://doi.org/10.17503/agrivita.v40i3.1296 498