Human Journals Research Article October 2017 Vol.:10, Issue:3 © All rights are reserved by Shraddha S. Ghodke Simultaneous Spectroscopic Estimation by Ratio Derivative Method of Metformin Hydrochloride and Gliclazide in Uncoated Bilayered Extended Release Combined Solid Dosage Form and its Application to Dissolution Studies www.ijppr.humanjournals.com Keywords: Metformin hydrochloride, Gliclazide, Bilayered Extended-release solid dosage form, Dissolution studies. ABSTRACT A simple, rapid, precise & efficient UV Spectroscopic method for simultaneous analysis of metformin hydrochloride and gliclazide in extended release combined tablet solid dosage form has been developed and validated. Practically no interference from tablet excipients was observed in these developed methods. As their λmax does not differ more than 20 nm, (232 nm for Metformin Hydrochloride and 229.5 nm for Gliclazide); hence, absorption corrected method was tried for their simultaneous estimation in extended-release tablet formulation. Quantitative estimation of GLZ was carried out by subtracting interference of MTF using experimentally calculated absorption factor. Both the methods are accurate, simple, rapid, precise, reliable, sensitive, reproducible and economical as per ICH guidelines. The values of % RSD and correlation of coefficient were satisfactory and results of the formulation analysis and result of the recovery study indicate that there is no interference due to excipients present in the formulation. It can be easily and conveniently adopted for routine quality control analysis. The absorption corrected method was successfully applied for the dissolution study. Validation revealed the dissolution method is specific, rapid, accurate, precise, reliable and reproducible. Shraddha S. Ghodke Department of Pharmaceutical Chemistry, Maharashtra Institute of Pharmacy, Faculty of Pharmacy, University of Pune, Maharashtra, India Submission: 22 September 2017 Accepted: 3 October 2017 Published: 30 October 2017