Int.J.Curr.Microbiol.App.Sci (2017) 6(6): 1493-1503 1493 Original Research Article https://doi.org/10.20546/ijcmas.2017.606.176 Xylanase and Phytase Supplementation in the De-oiled Rice Bran (DORB) based Diet Improves the Growth Performance of Labeo rohita Amit Ranjan, Narottam Prasad Sahu * , Ashutosh Dharmendra Deo, H. Sanath Kumar, Sarvendra Kumar and Kamal Kant Jain Central Institute of Fisheries Education, Mumbai, India *Corresponding author ABSTRACT Introduction De-oiled rice bran (DORB) is one of the major ingredient used in the fish feed. DORB is the by-product after extraction of oil from rice bran. At present about 7.0 MT of ingredients are being used for farm-made fish production (Ramakrishna et al., 2013). The current aquaculture production of the country (4.3 MMT) which is projected to reach 12.7 MMT by the year 2030 as reported by World Bank report (Msangi et al., 2013). Considering the total aquafeed production of India which is about 1 MMT as reported by Alltech (2014) is set to reach around 3 MMT by the year 2030 but the feed demand will be around 23 MMT hence the major part of feed will be the Mash feed that mainly contains DORB (about 80-85 %) (Ramkrishna et al., 2013). The carps together contribute to more than 82% of total aquaculture production in India. About 97% of the carp feeds used by Indian farmers are farm-made (Ramkrishna et al., 2013). But looking into the DORB production of India which is presently about 6 MMT is not going to increase to support the International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 6 (2017) pp. 1493-1503 Journal homepage: http://www.ijcmas.com A 60-day experiment was conducted to study the effect of xylanase and phytase supplementation in the DORB based diet on growth performance of Labeo rohita. Two iso-nitrogenous (crude protein-14%) test diets (T1-DORB based diet, T2- phytase and xylanase supplemented diet) were formulated. Sixty (60) juveniles of L. rohita, with an average weight 5.01±0.02g were stocked in six uniform size plastic rectangular tank in triplicates with 10 fishes per tank following a completely randomized design (CRD). At the end of the experiment growth performance and nutrient utilization of L. rohita were assessed and found that exogenous enzyme supplementation @ 0.01% in the DORB based diet significantly (p<0.05) improved the growth performance and nutrient utilization (weight gain % from 63.6% to 79.7%, SGR from 0.82 to 0.98, PER from 2.01 to 2.44 and FCR reduced from 3.57 to 2.92) of L. rohita. Carcass composition of fishes did not vary significantly. Hence, the experiment concludes that exogenous enzyme supplementation of xylanase and phytase in the DORB based diet improves the growth performance of L. rohita. Keywords De-oiled Rice bran (DORB), Labeo rohita, Xylanase, Phytase, Growth, Body composition. Accepted: 21 May 2017 Available Online: 10 June 2017 Article Info