_____________________________________________________________________________________________________ *Corresponding author: E-mail: drpriyanka0690@gmail.com; Journal of Pharmaceutical Research International 33(43A): 428-432, 2021; Article no.JPRI.73122 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) A Comparative Analysis of Penetration and Adaptation of Two Types of Light Cure Pit and Fissure Sealants: An In-vitro Study Garima Mukesh Sharma 1 , Jayashree Rajesh Patil 1 , Priyanka Paul Madhu 1* , Kumar Gaurav Chhabra 1 and Amit Reche 1 1 Department of Public Health Dentistry, Sharad Pawar Dental College and Hospital, DMIMS (Deemed to Be University), Sawangi (Meghe), Wardha 442001, Maharashtra, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i43A32508 Editor(s): (1) Dr. Juan Carlos Troiano, University of Buenos Aires, Argentina. Reviewers: (1) Weeraya Tantanapornkul, Naresuan University, Thailand. (2) A.Vasanthakumari, Dr.MGR Medical University, India. Complete Peer review History: https://www.sdiarticle4.com/review-history/73122 Received 22 June 2021 Accepted 28 August 20 India.21 Published 07 September 2021 ABSTRACT Background: Pit and fissure sealants are well-known for their ability to prevent occlusal caries. Given the difficulties of maintaining complete isolation for longer periods of time when treating young patients, a streamlined sealant application process is needed. As a result, the goal of the present study is to compare the penetration and adaption of 2 types of light cure sealants. Objectives: 1. To evaluate the adaptation and penetration of Conseal f ‘pit and fissure sealant’. 2. To evaluate the adaptation and penetration of PF seal SE ‘pit and fissure sealant’. 3. To evaluate comparison of adaptation and penetration between two types of light cure sealants. Methodology: This will be an in-vitro comparative study, which will consist of 2 categories - Group I (acid-etch fissure sealant, Conseal f) and Group II (self-etch fissure sealant, PF seal SE). A total of 20 extracted permanent teeth will then be classified into 2 groups of 10 teeth respectively and stereomicroscope will be used to compare the penetration and adaptation between two types of light cure pit and fissure sealants. Study Protocol