2274 Indian Journal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1 GC-Mass Spectrometry Analysis of Iraqi Moringa Oleifera Seeds Extract Fiham Jassim AL-Obaidi 1 , Abid A. Thaker 2 , Asmiet Ramizy 3 1 MSc. Student, 2 Prof. Department of Biology, college of Science, University of Anbar, 3 Prof., Department of Physics, college of Science, University of Anbar Abstract Moringa oleifera is an important traditional medicinal plant usedfor the treatment of many health problems. It is widely cultivated across the world but it has not well known orwell-studied in Iraq. Therefore, the aim of this study is to identify the bioactive compounds present in acetone:methanol (1:1) seeds extractof Iraqi Moringa olieifera by using GC-Mass Spectrophotometry. The results of this analysis revealed 41 compounds which include ,terpenes ( cis-beta-Ocimene 0.97%, Cedrene 0.20%, Cedrenol 1.40%, Verrucarol 0.29 , Dehydroxy-isocalamendiol 0.09% , beta-Myrcene 0.14% , 3-Thujene 0.06%, Ocimene 0.72% , Sabinene 0.37% Aromadendrene 1.54%) , phenols (0.34%, Eugenol 0.49%, cis-Isoeugenol 1.21%, Methoxyeugenol 0.26%, Ferrocene 0.57%, Phenol, 2-methoxy-4- (1-propenyl)-, (Z) 0.34 , Oxabicyclo[3.3.0]octan-2-one -7-neopentylidene) 0.61% ) , fatty acid methyl esters ( n-Tridecanoic acid 0.15% , Undecanoic acid 0.09% , Pentadecanoic acid 34.43%, 4-Hexadienal 1.52% , Dodecanoic acid 11.02% , cis-Vaccenic acid 21.39%, Hexadecenoic acid 0.15% , Eicosanoic acid 0.03%), acid (myristicin,7.8%), Phenyl propanoid (Asarone, 4.99%), Antioxidant (-tert-Butyl-4-hydroxyanisole, 1.38%), Drug (Berbine, 2,3,9,10-tetramethoxy, 0.73%), Drug (Thiazolo, 0.63%), aldehyde (Trimethylsilyl vanillin, 0.61%), hormone (Octenoic acid, 0.46%), Amine (ethyl ester, 0.42%), Carboxylic acid (Dimethylandrost- 5-en-3-one, 0.28%), Terpenoid (Lavandulol, 0.19%), Heterocyclic compound (Buten-3-one, 1-(2-carboxy- 4,4-dimethylcyclobutenyl, 0.18%), Volatile oil (Butanoic acid, 0.16%), Fatty alcohol (1-Heptatriacontanol, 0.12%), Hydroxy carbon-alkene-(Carene, 0.11%). It can be concluded from this study that the seeds are considered a rich source of bioactive compounds. Keywords: GC-MS analysis, Bioactivecompounds, Methanol:Acetone Extract, Iraqi Moringa oliefera. Corresponding author: Fiham Jassim AL-Obaidi drabidthaker@gmail.com Introduction Medical plants are commonly used as a source of medicinal products or against the toxicity of xenobiotics (1,2,3) . World Health Organization reports that 4 billion people (80 percent of the world’s population) use herbal medicinal goods for most of the primary health care (4) . Moringa oleifera is among 13 species of Moringa trees that grow in different countries around the world (5) . Seeds of M. oliefera tree are useful for medicinal, practical food preparations, water purifcation, and biodiesel production. (6) . M. oliefera seeds are a good source of proteins, lipids, vitamins, minerals, micronutrients, and bioactive phytochemicals (7,8,9 ) . The aqueous seeds extract of M. oliefera inhibit bacterial and fungal growth (10 ) . The methanol: acetone seed extract of Iraqi M. oliefera is found to reduce the toxicity of nanoparticles of lead in the rat (Unpublished results). This study aims to estimate the compounds found in methanol: acetone seeds extract of Iraq M. oleifera by using GC mass spectroscopy.