Research J. Pharm. and Tech. 12(1): January 2019 227 ISSN 0974-3618 (Print) www.rjptonline.org 0974-360X (Online) RESEARCH ARTICLE Physiochemical and Acute Toxicity Analysis of a Siddha Herbo-Mineral Formulation – Pachai Karpoora Vadagam (PKV) Dr. Shunmugaram S * Research Officer (S), Siddha Regional Research Institute, Puducherry. *Corresponding Author E-mail: dr.s.s.ram28@gmail.com ABSTRACT: Siddha system of medicine is one of the traditional systems of medicine which belongs to southern part of India. It widely uses herbs, metals and minerals as raw drugs for medicine preparation. The medicine which possesses metals and minerals are better to undergo physiochemical and toxicological analyses that help us to prove our drugs are nontoxic in nature. Pachai Karpoora Vadagam (PKV) is a Siddha Herbo-mineral formulation, prepared as per the Siddha literature Gunapadam Thaathu Seeva Vagupu. The phytochemical and physiochemical analysis like colour, odour, solubility, ash values and pH analysis were analyzed. The acute toxicity study was done in Swiss albino rats. The physiochemical of PKV revealed that all parameters were within normal range. The toxicity study did not exhibit any mortality in rats. No weight reduction and behavioral changes was noted. All internal organs were found to be normal in necropsy. Hence the Siddha formulation PKV is safe for human usage. KEYWORDS: Siddha Medicine, Herbo-mineral, Pachai Karpoora Vadagam, Physiochemical analysis, Toxicity study. 1. INTRODUCTION: Siddha system of medicine is one of the ancient systems contemporaneous with those of submerged lands, Egyptian, Mesopotamian, Chinese and Grecian medicines. The unique nature of this system is, its continuous service to humanity for more than 5000 years in combating diseases and in maintaining its physical, mental and moral health while many of its contemporaries had completed their course. The word ‘Siddha’ means establishing truth. The pharmacopoeia of Siddha system contained large number of plants, metals, minerals and animal products. The purification, combinations, measurement and the methods of preparations are given with minute precision in Siddha literature. 1 Pachai Karpooram (Borneo Camphor) is one among the Kaara Saaram (Minerals) which is widely used in Siddha preparation for many years. Received on 23.08.2018 Modified on 17.10.2018 Accepted on 21.11.2018 © RJPT All right reserved Research J. Pharm. and Tech 2019; 12(1): 227-230. DOI: 10.5958/0974-360X.2019.00042.8 The drug Pachai Karpoora Vadagam (PKV) is a herbo- mineral preparation which consist single mineral Pachai Karpooram and 4 herbs. It has high therapeutic value. It is used to treating the diseases like Upper respiratory tract infection, Arthritis etc. 2 Standardization of Siddha mineral drugs in order to assess the quality was based on the concentration of their physical, chemical, phytochemical standardization. Hence the purification process, may reduce the toxic nature of the drug it is mandate now a days to ensure the safety of the finished product by conducting toxicity studies according to standard guidelines. It should ensure the safety and efficacy of the classical text formulation before human usage. 2. MATERIALS AND METHODS: Pachai Karpoora Vadagam has selected from the Siddha literature “Gunapadam Thaathu Seeva Vagupu” page no.418. 2.1 Ingredients 2 : The test drug PKV contains Pachai Karpooram (Borneo Camphor) 35 grams, Santhana Thool (Powder of Santalum album Linn.) 70 grams, Elam (Elettaria