(IJIRSE) International Journal of Innovative Research in Science & Engineering ISSN (Online) 2347-3207 IJIRSE/Vol 3. Iss. 3/ Page 74 Invigoration and Root Growth Enhancement of Mung Bean Seeds Through Pre-Treatment With Allium Cepa 30C – An Agrohomeo Study Anindita Dey 1* , Ruma Basu 2 , Sukhen Das 3 , Papiya Nandy 1 3 Centre for Interdisciplinary Research and Education, 404B Jodhpur Park, Kolkata 700068 2 Physics department, Jogamaya Devi College. Kolkata 700026, India 32 Physics Department, Jadavpur University. Kolkata 700032, India Abstract:-Keeping the seed health well during storage is a very serious problem as this makes old seeds incapable to fully germinate. We report here for the first time that pre-treatment of two year-old mung bean seeds with a low cost homeopathic medicine Allium cepa 30C is effective under normal laboratory conditions in invigorating the seeds as realised by their higher growth rate, as well as enhancement of the root growth from these seeds. We also showed that the medicine does not have any side effect, thus making this study very useful for farmers in the horticultural as well as agricultural sectors. Key words: Invigoration, root growth, pre-treatment, viability, Seed electrolyte, Allium cepa I. INTRODUCTION Maintenance of seed viability during storage is a serious problem the horticultural as well as agricultural sectors face in the tropical countries. During storage, high temperature and relative humidity accelerates the deterioration of seed membrane through loss of water from membrane tissues which in turn damage the cells [1]. Ascorbic acid regulates the hydrogen peroxide [H2O2] level in water stressed tissues. H2O2 is a toxic metabolite which is accumulated in water stressed tissues and increase membrane permeability [2]. Over the years various methods have been adopted by scientists like hydration-dehydration techniques as well as treatment of seeds with chemicals, vitamins, fungicides, plant growth regulators, antioxidants etc [3-7]. However, farmers prefer to choose the techniques which are easy, cost effective, non toxic or non hazardous and do not take much time. . We realize that homeopathic medicines are universally used for centuries, because of their negligible side effects, low cost, easy availability and easier applicability [8-13]. The effects of homeopathic medicines have been studied in details mostly in animal systems [14-17]. However, plants being the silent receptors of the ecosystem, we were interested to find the effect of homeopathic medicines specially on invigoration of plants. Recently researchers are engaged themselves in the field of agrohomeo studies [18-22]. In the present study we have used for the first time a homeopathic medicine, Allium cepa 30C, a well known homeopathic medicine, commonly used for treatment of cold, to study its effect on two year old mung bean seeds. Our experiments conducted on petriplate using mung bean seeds, pre-treated with Allium cepa 30C, indicated that the drug improved the seed membrane health and showed that they not only could invigorate the seeds as realised by their higher growth rate, but they also enhanced the root growth from these seeds. We also showed that the medicine does not have any side effect, thus making this study very useful for farmers. II. MATERIALS & METHODS: A. Seed collection: Certified two-year old mung bean seeds were collected from a local farm of Hooghly District. The germinability of the seeds was 65% on the average.