www.tjprc.org editor@tjprc.org DESIGN AND CREATION OF VAGINAL LACTIC ACID BACTERIA ASSOCIATION RESISTANT TO PHYSIOLOGICAL CYCLIC PH DISCHARGES GOHAR G. GRIGORYAN 1 & HRACHYA G. HOVHANNISYAN 2 1 Yerevan State Medical University after M. Heratsi and “Arm Biotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia 2 “Arm Biotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia ABSTRACT Aim: The aim of this study is creation of sustainable consortium of symbiotic probiotic bacteria with high colonization potential for rapid response to vaginal acute and chronic challenges. Material and method: In this study used biocompatible lactobacilli and cocci possessing high antimicrobial activity, isolated previously from vaginal samples of healthy women from 20 to 45 years old from Armenia. The following methods: screening of antimicrobial activity, microbial adhesion to solvents, auto aggregation and co aggregation assays were used Statistical analysis used: Student's test computer, taking the criterion of P <0.05 sufficient for significant differences in the results. Results: The symbiotic consortium of Lactobacillus acidophilus GH 201 and Lactococcus lactis GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans, intensive growth rate and biomass accumulation, than the single cultures The consortium is sustainable during multiple sub culturing imitating up and down changes of vaginal pH. Conclusion: The sustainable consortium of symbiotic probiotic bacteria have high colonization potential for rapid response to vaginal cyclic pH discharges caused by menses and frequently intercourses. These bacteria possess enhances growth rate, antimicrobial and adhesive effects, when working together. KEYWORDS: LABS Consortium, Antimicrobial Activity, Adhesion, Auto aggregation, Co Aggregation Received: Jul 07, 2016; Accepted: Aug 02, 2016; Published: Aug 09, 2016; Paper Id.: IJMPSAUG20164 INTRODUCTION Lactic acid bacteria (LAB) are considered the dominant microflora of healthy woman's vagina, they produce lactic acid lowering vaginal рН < 4.5, hydrogen peroxide (H 2 O 2 ), bacteriocins and other antibacterial substances that protect from sexually transmitted infectious [1-3]. Various factors; nutrition, hygiene, stress, infections, low immunity, etc., destroyed vaginal microbial balance, which dramatically reducing lactobacilli and brings to propagation of resident anaerobe microorganisms, which cause bacterial vaginosis [4]. The vaginal ecosystem is sensitive to disturbances caused by menses and human activities such as frequently intercourses, douching, using of antibiotics for non-vaginal illnesses and other habits and practices. Over the course of the menstrual cycle, vaginal levels of hormones and glycogen vary, and menstrual blood (pH 7.32) alters vaginal pH Original Article International Journal of Medicine and Pharmaceutical Science (IJMPS) ISSN(P): 2250-0049; ISSN(E): 2321-0095 Vol. 6, Issue 4, Aug 2016, 27-34 © TJPRC Pvt. Ltd.