Plant Archives Vol. 19 No. 2, 2019 pp. 3173-3180 e-ISSN:2581-6063 (online), ISSN:0972-5210 *Author for correspondence : E-mail : akeelalassie@gmail.com MOLECULAR IDENTIFICATION AND BIOLOGICAL RESISTANCE OF THE FIG MOSAIC VIRUS ( FMV ) ON FIG TREES IN SALADIN GOVERNORATE NURSERIES Raghda J. Mohmmed 1 , Akeel H. AL Assie 1 * and Maadh A. Al Fahad 2 1* Department of Biology, Collage of Science, University of Tikrit, Iraq. 2 Department of Plant Protection, College of Agriculture, University of Tikrit, Iraq. Abstract The study aimed at diagnostic FMV virus from infected Ficus carica from some Saladin nurseries based on the technique of Reverse Transcription RT-PCR. A band 300 bp was given. The result showed FMV similar to that found in Costa Rica, Iran, Lurstan, Japan, and also gave a band of 367pb of FMV RNA1 and the absence of other species. FLMAV-1 isolation was detected in fig leaf with a 352pb band and is similar in some countries. The study also included the use of Bacillus subtilis, Spirulina platensis, Ganoderma lucidum, (G. lucidum +S. platensis), (S. platensis+B. subtilis), (G. lucidum+B. subtilis). The treatment of B. subtilis + S. platensis for yellow and black to study the percentage of the severity of the effect of the injury which is the lowest and reached 23.0% compared with a control treatment, which gave 99.6%. The results of chlorophyll the treatment of mushrooms with moss (G + Sp) with a value of Spad 43.15 which is the highest compared to the treatment of control amounted to 30.77 Spad. Key words : FMV, FMV RNA1, RT-PCR, Ficus carica. Introduction Ficus carica is one of the most ancient fruit trees grown in the Middle East. It is needed for its nutritional and medical value (Flaishman et al., 2008). It has a high content of natural compounds including beta-carotene- producing vitamin A which has anti-oxidant and anti- Cancer and the beta-cytosterol compound, known for its ability to lower blood cholesterol, as well as the presence of a number of effective plant compounds, such as Glycosides, Arabinose and Xanthotoxol (Abdel Nasser and Mohammed, 2014). As for the area planted with figs in the Arab world, it is estimated at 155000 thousand hectares and the number of trees is 428037 thousand trees and the production is 72514 thousand tons. In Iraq, the number of trees planted 416 thousand trees and the production rate of 10.00 thousand tons, which was the latest statistic for 2013 (Arabian organization for nutritional growth 2014). The FM Virus disease is one of the most common viral diseases in fig trees in the cultivated areas of the world, causing large losses and different on the leaves and fruits and reduce the production in quantity and quality (Anofka et al., 2000). This virus is due to the taxonomic order of the genus Emara virus, the family of Fimovirideae and the rank of Bunyavirale (Ishikawa et al., 2013) has four or six parts of the genomic RNA (Walia, et al., 2014). These viruses are common in the way they are transmitted by different types of ways by known mechanism as well as about 700 types Of the Moraceae family, including figs transmitted by this mechanism (Failshman et al., 2008: Datwyler and Weilblen, 2004). The virus was found in most fig trees in Iraq (Shawkat, 1982). The virus spreads widely in the Middle East and has been recorded in several countries, including Tunisia and Egypt (Nahdi et al., 2006), as well as in Japan, Costa Rica, Croatia, Turkey, China, Iran and other countries. New methods of resistance to viruses were used with natural dietary supplements and were effective because the plants had defensive means that could be biologically stimulated using supplementation as well as with the protective side, decrease in infection criteria and increase in growth indicators (Youssef, 2018). (Saliva et al., 2002, Diwan: 2003). The serological tests were then