Received on: 16/07/2014 Accepted on: 30/09/2014 Published on: 15/10/2014 Shruti Rastogi Department of Phytopharmaceuticals, Indian Pharmacopoeia Commission, Ministry of Health & Family Welfare, Sector 23, Raj Nagar, Ghaziabad (U.P.), India Email: rastogishruti87@gmail.com QR Code for Mobile users DOI: 10.15272/ajbps.v4i36.554 Development and Validation of a Liquid Chromatography Method for Determination of Embelin in Crude Extract of Embelia ribes Shruti Rastogi*, Amandeep K Bhatia, Ashish Kushwaha, Manoj K Pandey, Alok Sharma, Jai Prakash, G.N. Singh * Department of Phytopharmaceuticals, Indian Pharmacopoeia Commission, Ministry of Health & Family Welfare, Sector 23, Raj Nagar, Ghaziabad (U.P.), India – 201002. Abstract This study was designed to develop and validate a liquid chromatographic method for determination of Embelin in crude extract of Embelia ribes. The chromatographic separation was performed on a HPLC coupled to a diode array detector with Thermo Hypersil-Keystone, BDS C-18 column (25 cm L x 4.6 mm ID; particle size- 5μ) using methanol and 0.1% TFA (88:12 v/v) as mobile phase, at a flow rate of 1 ml/min. Quantification was achieved with UV detection at 288nm, based on peak area. The optimized procedure involved the extraction of Embelin in crude extract of Embelia ribes by soxhlet and maceration using chloroform and methanol as a solvent. Extraction by soxhlet at 70°C for 2 hr using chloroform as solvent was the best suited method. The assay method was developed and validated for system suitability, linearity, range, accuracy, precision and robustness. The plot of integrated peak area and concentration of Embelin was found to be linear over a range of 6.25-200 µg/ml. The LOD and LOQ (µg/ml) levels were, 1.5 and 4.5, respectively. The recovery experiments led to mean recovery of 98.6%. This indicates that this method is simple, rapid, sensitive, precise and accurate and can be applicable for the extraction and quantitative determination of Embelin in crude extracts of Embelia ribes. Keywords: Extract of Embelia ribes, Embelin, HPLC. Cite this article as: Shruti Rastogi, Amandeep K Bhatia, Ashish Kushwaha, Manoj K Pandey, Alok Sharma, Jai Prakash, G.N. Singh. Development and validation of a liquid chromatography method for determination of embelin in crude extract of Embelia ribes. Asian Journal of Biomedical and Pharmaceutical Sciences; 04 (36); 2014, 9-13.