©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (3): 1707-1718 (2011) Review Application of RNA-seq to reveal the transcript profle in bacteria A.C. Pinto 1 , H.P. Melo-Barbosa 2 , A. Miyoshi 1 , A. Silva 2 and V. Azevedo 1 1 Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil 2 Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil Corresponding author: V. Azevedo E-mail: vasco@icb.ufmg.br Genet. Mol. Res. 10 (3): 1707-1718 (2011) Received July 7, 2011 Accepted August 4, 2011 Published August 16, 2011 DOI http://dx.doi.org/10.4238/vol10-3gmr1554 ABSTRACT. The large number of microbial genomes deposited in databanks has opened the door for in-depth studies of organisms, including post-genomics investigations. Thanks to new generation sequencing technology, these studies have made advances that have lead to extraordinary discoveries in bacterial transcriptomics. In this review, we describe bacterial RNA sequencing studies that use these new techniques. We also examined the advantages and biases of these new generation technologies; advances in bioinformatics make it possible to overcome the biases, providing interesting and surprising results. Key words: RNA-seq; Transcriptome; Bacteria