Costantine F. Daher et al. ——————————————————————————————————————————————————— WWW.SIFTDESK.ORG 442 Vol-7 Issue-2 SIFT DESK Accepted Date: 18 th Feb 2022; Published Date:25 th Feb 2022 Maria George Elias a , Yves Najm Mrad a , Bilal Nehmeh a , Carole Dagher c , Mohamad Mroueh b , Robin I. Taleb a , Cos- tantine F. Daher a,* a Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, P.O. Box 36, Byblos, Leba- non. b School of Pharmacy, Department of Pharmaceutical Sciences, Lebanese American University, P.O. Box 36, Byblos, Leb- anon. c School of Medicine, Lebanese American University, Byblos, Lebanon CORRESPONDENCE AUTHOR Costantine F. Daher E-mail: cdaher@lau.edu.lb Phone: 009613998985 Fax: 0096195466262 CITATION Maria George Elias, Yves Najm Mrad, Bilal Nehmeh, Carole Dagher, Mohamad Mroueh, Robin I. Taleb, Costantine F. Daher, Wild Daucus Carota Oil Extract: An Innocuous Preventative and Chemotherapeutic Agent Against Chemically Induced Breast Cancer (2022) Journal of Food Science & Technology 7(2) :442-455 ABSTRACT Daucus carota L. ssp. carota oil extract (DCOE) was shown to possess potent antitumor activity. This study investigates the chemopreventive and chemotherapeutic effects of DCOE against breast cancer induced by 7,12-Dimethylbenz(a) anthracene (DMBA) in rats. In the chemopreventive study, animals were pre-treated with DCOE (25 mg/kg; 1 week) fol- lowed by 14 weeks of treatment post-cancer induction with DMBA. In the chemotherapeutic study, animals were allocat- ed into control, DCOE (25 mg/kg) and Cisplatin (2.5mg/kg) groups. When tumor size reached a diameter of 14-15 mm, the animals were divided into three groups each injected by either a vehicle (thrice a week), DCOE (thrice a week) or Cis- platin (twice a week), for 8 weeks to compare and assess the therapeutic value of DCOE with respect to Cisplatin and the vehicle. At 24 weeks, the experiment was terminated. DCOE pre-treatment protected against DMBA-induced toxicity and reduced tumor incidence. While 18% of control died few days post DMBA treatment, 100% survival in DCOE pre-treated group was observed just before week 8. In the chemotherapeutic experiment, treatment with either DCOE or Cisplatin caused significant inhibition of tumor growth. Unlike DCOE, Cisplatin caused drastic decrease in body weight with 75% and 100% death rates at week 6 and 8 respectively. DCOE produced a significant increase in Bax/Bcl2 ratio, Cytochrome c and cleaved caspase 3 proteins. DCOE may be considered a safe chemopreventive and/or chemotherapeutic agent, an effect that is partly due to the induction of the intrinsic apoptotic pathway. Keywords: Daucus Carota, Wild carrot,  -2-Himachalen-6-ol, 7,12Dimethylbenz(a)anthracene, Breast cancer, Apopto- sis Abbreviations: DCOE, Daucus Carota Oil Extract; DMBA, 7,12-Dimethylbenz(a)anthracene; H&E, Haematoxylin and Eosin Copy rights: © 2022 The Author(s). Published by Sift Desk Journals Group This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any me- dium, provided the original work is properly cited. Wild Daucus Carota Oil Extract: An Innocuous Preventative and Chemotherapeutic Agent Against Chemically Induced Breast Cancer Journal of Food Science & Technology (ISSN: 2472-6419) DOI: 10.25177/JFST.7.2.RA.10791 Research