American Journal of Microbiology and Biotechnology 2018; 5(1): 12-17 http://www.aascit.org/journal/ajmb ISSN: 2375-3005 Keywords Azadirachta indica, Anopheles Mosquito, Larvicidal, Seed and Leaf Crude Extract, Environment, Bioinsecticides Received: November 16, 2017 Accepted: November 23, 2017 Published: April 10, 2018 Larvicidal Activity of Crude Seed and Leaf Neem Extracts (Azadirachta indica) Against Mosquito Larvae in Kogi, North Central, Nigeria Adobu Ugbede Shadrach 1 , Odoh Chuks Kenneth 2, * , Akpi Uchenna Kalu 2 , Anya Francis 3 1 Department of Biology, Kogi State College of Education, Anpka, Kogi State, Nigeria 2 Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria 3 Department of Biological Science, Abubakar Tafawa Balewa University, Bauchi, Bauchi State, Nigeria Email address * Corresponding author Citation Adobu Ugbede Shadrach, Odoh Chuks Kenneth, Akpi Uchenna Kalu, Anya Francis. Larvicidal Activity of Crude Seed and Leaf Neem Extracts (Azadirachta indica) Against Mosquito Larvae in Kogi, North Central, Nigeria. American Journal of Microbiology and Biotechnology. Vol. 5, No. 1, 2018, pp. 12-17. Abstracts The study was carried out to investigate the larvicidal potential of seeds and leaf crude extract of A. indica as an environmentally safe measure to control malaria vector. The early fourth-instar larvae of anopheles were reared in the laboratory. At varying level of larvae exposure to the crude seeds extract of A. indica; 1ml, 5ml, 10ml, 15ml and 20ml. 30%, 55%, 73%, 92% and 100% mortality rate were recorded at interval of 72 hours with LC 50 value (lethal concentration) of 4.4ml while the crude leaf extract presented 32%, 56.5% 76%, 86% and 100% mortality and LC 50 4.5ml respectively. The result of the crude seed extracts of A. indica when compare to that of leaf extract were found to be an effective bioagent in controlling the propagation of anopheles larvae as there was no significant different (p < 0.05) in the level of their larvicidal activity and severity. The result therefore suggest A. indica as a sound candidate for the formulation of bioinsecticides to curb rising malaria scourge within the tropics while minimizing the cost and over dependence on synthetic insecticides that has long damaging effect on the environment. 1. Introduction Azadirachta indica commonly known as Chinaberry or Persian lilac tree is of maliaceae plant family containing variety of compounds that show insecticidal, antifeedant, growth- regulating and development-modifying properties [1, 2]. Being a native to northwestern India, the trees also grow in the tropical and subtropical parts of Asia. Of recent, through biotechnology and their climatic tolerance, neem tree has been successfully grown in other warm region of the world especially in the tropics and sub Saharan Africa. Previous works has implicated fruit extract of A. indica as an antifeedant, moulting disorders, growth retardation, reduced fecundity, morphogenetic defects, and changes of behaviour agent. According to [3, 4], fruit extracts of Melia azedarach and A. indicae licit some morphogenic, physiological and behavioural changes in insects.