Published online in http://ijam. co. in 52 Meena Shamrao Deogade et.a.l., Standardization of wild Krushnatulasi (Ocimum tenuiflorum Linn) Leaf ISSN: 0976-5921 Standardization of wild Krushnatulasi (Ocimum tenuiflorum Linn) Leaf Meena Shamrao Deogade 1 , Prasad KSR 2 .1. Professor Department of Dravyaguna Mahatma Gandhi Ayurved College Hospital & Research Centre, Salod (H), Wardha. M.S. 2. Professor & Head Department of Panchakarma, YMT Ayurvedic Medical College & Hospital. P.G.Institute, Kharghar, Navi Mumbai, MS , India. Abstract Background: For acceptance and globalization of Ayurveda there is needed to analyze herbal drugs according to modern techniques. Assessment of complete and accurate pharmacognostical study of herbs used in Ayurveda provides scientific basis of its quality. Objectives: To standardize the Kushnatulasi (Ocimum tenuiflorum Linn/ Ocimum sanctum L ) collected from wild. Materials and Methods: The present study includes organoleptic, microscopic physicochemical, phytochemical and chromatographic examination of leaf of Kushnatulasi. Results: macro and microscopic, organoleptic, physicochemical, phytochemical and chromatographic findings are observed as per API in present study. Conclusion: Standardization of Krushnatulasi (Ocimum tenuiflorum L inn) is useful in authentication of genuine drug. Keywords: Krushnatulasi, Ocimum tenuiflorum Linn, Ocimum sanctum L, Standardization, wild, Pharmacognostic. *Corresponding Author: Meena Shamrao Deogade, Professor, Department of Dravyaguna, Mahatma Gandhi Ayurved College Hospital & Research Centre, Salo (H), Wardha (MS), E-mail: drmmeena@rediffmail.com Introduction In ancient Ayurveda texts the concept of standardization and quality control of drugs is found. Physician used to collect the drugs himself with the help of Shabda (sound), Sparsha (texture), Roopa (color), Rasa (taste), Gandha (smell) and also based on habitat, morphology etc. in those days. After checking all these factors the drug would be used as a medicine. The nomenclature of many herbs denotes their physical, chemical characteristic and therapeutic uses which are considered as primitive standardization parameters (1). Surasa, Sulabha, Surabhee, Shulaghnee, Bahumanjaree, Bhutaghni these synonyms of Krushnatulasi depicted its useful form (juice) in many diseases, easy availability, aroma, efficacious in colic, morphological character and antimicrobial action respectively. In current period recent advances has identified many test and parameters to evaluate quality control of drugs by pharmacognostic studies. So it is very essential to lay down pharmacognostic study of medicinal plants which are used in various formulations. It deals with authentication and standardization of natural drugs. Authentication and standardization evaluated by morphological or organoleptic tests, microscopic, chemical, and physical evaluation, chromatography, spectrophotometry etc. Pharmacognostic study includes parameters which help in identifying drug in dry powder form also. This is again necessary because once the plant is dried and made into powder form, it loses its morphological identity (2). Therefore it is necessary to provide standard parameters for the quality control of Ocimum tenuiflorum L inn (Krushnatulasi) leaves which can be beneficial for further quality control researches. Krushnatulasi (Ocimum tenuiflorum L inn) or Queen of herbs has been used in Ayurveda for its varied healing properties. Krushnatulasi the Holy basil, the legendary ‘Incomparable one’ of India, is one of the holiest and most cherished of the many healing and healthy giving herbs of the orient (3). Materials and Methods Sample Collection The leaves of Ocimum tenuiflorum Linn (Krushnatulasi) were collected as per GPS co-ordinator during the period of 21 July to 30 September 2015 from village Dabha, Wardha District (M.S.). These leaves were collected from open shrub land. Authentication The herbariums of Ocimum tenuiflorum Linn was prepared by standard method (4, 5) and sent for identification to Botanical Survey of India, Pune, Maharashtra. Research Article