Nat. Volatiles & Essent. Oils, 2021; 8(6): 2521-2530 2521 Improving Shoot Multiplication Of Strawberry (Fragaria Ananassa L. Cv. Roby Gem) In Vitro By Using Agnps And Iron Nanoparticles Husham K. Abbas 1,2 and Muslim A. A. Abdulhussein 1* 1 Directorate of Agriculture of Al-Najaf Governorate, Ministry of Agriculture, Iraq. 2 Department of Horticulture and Landscape, Faculty of Agriculture, University of Kufa, Najaf, Iraq. ABSTRACT The current study was carried out to study the effect of adding different concentrations of nano silver nitrate (0, 5, 10 and 15 mg L -1 ) to the cultures in initiation stage of strawberry (Fragaria ananassa cv. Ruby Gem) and adding the same concentrations of iron (traditional and nano iron) on shoot multiplication stage. Results showed that there were significant differences between nano silver nitrate concentrations in initiation stage as it increased the response percentage, number and length of shoots and reduced the percentage of contamination of cultures at 10 and 15 mg L -1 concentrations compare to the control treatment (0 mg L -1 ). The outcome of shoot multiplication experiment indicated that silver nitrate concentration and the source of iron separately had significant positive effect on studied traits as well as their interaction, when the 10 mg L -1 of nano silver nitrate and nano iron treatment gave the highest average of shoots number and its dry and fresh weight as it reached 11.00 shoot, 2.23g and 0.26g respectively in comparison with lowest average recorded 5.33 shoot, 1.28g and 0.16g respectively in control treatment with traditional iron. Key words: strawberry, nano silver nitrate, traditional iron, initiation stage, shoot multiplication. INTRODUCTION The cultivation of strawberry (Fragaria ananassa L. Duch) is spreading in more than 64 countries and its productivity amounted 9.22 million ton globally (FAO 2017, and many arab countries are cultivated it widely particularly Egypt, Syria, Palestine and Lebanon. However, the cultivation of this vegetable crop is limited in some areas, scientific experiment stations and gardens, with low consuming rate and most strawberry are imported from Syria, Iran and Turkey. Strawberry is reproduced by two main methods, sexual method when the seeds are used to produce new cultivars but this method is not preferred due to different varieties that produced from original plant and low germination percentage of its seeds. Therefore, many growers use the asexual method (vegetative method) widely by dividing plants that produce few rhizoid as this method is considered the most dominant to produce strawberry commercially (Mohan et al., 2005; Neamah et al., 2020). One of the main challenges that facing plants cultivated by vegetative method is the infection with one or more plant pathogens especially viruses which transferred from infested to healthy plants by aphid insects and lead to reduce the growth and yield. Many attempts has been done to reproduce the important