JOURNAL OF IRANIAN CLINICAL RESEARCH * Corresponding author: Homa Soleimani, Department of Medical Physics and Physiology, School of Medical Sciences, Arak University of Medical Sciences, Arak, Iran, Email: dr.hsoleimani@arakmu.ac.ir ORIGINAL ARTICLE INTRODUCTION The influence of static magnetic field (SMF) on biological system has been the topic of considerable interest for many years [1]. The moderate intensity of SMF (i.e., 1 mT-1 T) is not ionizing and do not exert any thermal effects on the biological systems. This intensity has potentially deep penetration into different tissues. Fur- thermore, it affects the macromolecule performance and behavior, such as calcium influx, ion transfer, membrane potential differences, radical production, life span, and eventually oxidative stress [1, 2]. The exact mechanism of SMF is still unclear due to differences in the intensity, cell, and time exposure. One of the notable findings of SMF is changing the rate of cell death (apoptosis) [3, 4] and viability. To find out these parameters on cells, the rate of cell death and amount of DNA damage have been evaluated in the presence and absence of SMF and various anticancer drugs [2]. Cisplatin has been used as the first-line therapy for several cancers. This medication can induce apoptosis following DNA damage like many other chemotherapeutic drugs [5]. Anticancer drugs have cytotoxic effects on both normal and cancerous cells. To reduce the associated side effects, a variety of unified treatment is required. Magnetic fields presu- mably provide a new strategy for the effective treatment of cancers and reduce the side effects of antineoplastic drugs [6]. With this background in mind, the present Effects of Static Magnetic Fields on Viability and Apoptosis in Normal and Cancerous Cells Samaneh Kamalipooya 1 , Homa Soleimani 2* , Parviz Abdolmaleki 3 , Amir Sabet 3 , Behnam Hajipour 3 , Fatemeh Javani Jouni 3 1 Department of Biotechnology, School of Medicine, Arak University of Medical Sciences, Arak, Iran 2 Department of Medical Physics and Physiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran 3 Department of Biophysics, School of Bioscience, Tarbiat Modares University, Tehran, Iran ABSTRACT Background: The influence of static magnetic fields (SMFs) on living organisms has been the topic of considerable interest for many years. However, the exact mechanism of SMFs is still unclear. Regarding this, the present study was conducted to evaluate the possible relationship between SMF and cancer treatment and also determine the possible effects of co-treatment with anticancer drugs on normal and cancerous cells. Materials and Methods: The effects of 10 mT SMF on cell death (sub-G1 and apoptosis/necrosis) were investigated using the flow cytometric methods. The SMF was utilized both in the presence and absence of cisplatin as an anticancer agent in the HeLa cell line and Hu02 as cancerous and normal cell types, respectively. Results: According to the results, the SMF exposure caused an increase in cell death in Hu02 24 h post-treatment and HeLa cell line both 24 and 48 h after treatment. Moreover, the co-treatment of SMF and cisplatin led to an enhancement in cell death within the first 24 h of treatment via necrosis as well as early and late apoptosis. On the other hand, this co-treatment resulted in the reduction of cell death in the first 48 h post-treatment in both cell types. Conclusion: Even though 10 mT intensity of SMF was not a high value, it was able to change the cell function and structure, which in turn led to a change in apoptosis rate. The findings of the present study might indicate that the SMF could decrease the cell death. Nevertheless, it is essential to perform more investigations to find the exact related mechanisms. Key Words: Apoptosis, Cisplatin, Flow cytometery, HeLa cell line, Static magnetic field, Sub-G1  How to cite this paper: Kamalipooya S, Soleimani H, Abdolmaleki P, Sabet A, Hajipour B, Javani Jouni, F. Effects of Static Magnetic Fields on Viability and Apoptosis in Normal and Cancerous Cells . Journal of Iranian Clinical Research. 2015; 1(3): 81-85.