S-1 High-throughput discovery of aptamers for sandwich assays Andrew T. Csordas 1† , Anna Jørgensen 1† , Jinpeng Wang 1† , Emily Gruber † , Qiang Gong † , Elizabeth R. Bagley † , Margaret A. Nakamoto § , Michael Eisenstein † , H. Tom Soh †* † Institute for Collaborative Biotechnologies, University of California at Santa Barbara, Santa Barbara, CA, 93106, USA § Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, 94305, USA Contents Supplemental Methods …………………………………. S-2 – S-6 PAI-1 detection limit calculation ………………………. S-7 Figure S1 ………………………………………….……. S-8 Figure S2 …………………………………….…………..S-9 Figure S3 ……………………………………….………..S-10 Table S1 ………………………………...……………… S-11 References ………………………………………………S-12