757 Published by Bali Medical Journal | Bali Medical Journal 2021; 10(2): 757-762 | doi: 10.15562/bmj.v10i2.2524 ORIGINAL ARTICLE ABSTRACT Epidermal Growth Factor (EGF) representing the role of other growth factors contained in Platelet-Rich Plasma (PRP) Dessyta Sukma Nurmaulinda 1* , Agus Santoso Budi 1 , Lobredia Zarasade 1 , Budi Utomo 2 Background: Wound care with modern dressings that have been widely used takes time to achieve healing. Therefore, Epidermal Growth Factor (EGF) and Platelet-Rich Plasma (PRP) are developed in wound healing therapy. EGF as the primary growth factor examines whether it is sufcient to represent the role of other growth factors in PRP. EGF is also chosen because of its ease of provision and longer shelf life compared to PRP. This study evaluates the (EGF) representing the role of other growth factors contained in PRP. Methods: The study was performed on 66 full-thickness wounds in 6 groups of 36 healthy male Oryctolagus cuniculus rabbits. Four treatment groups were given EGF and PRP therapies. Two control groups were given no treatment. Half of the groups were evaluated on the ffth day and the rest on the fourteenth day. Assessment on the clinical macroscopic and histopathological numbers of fbroblasts, capillary blood vessels, and type III collagen fbers were stained with Hematoxylin Eosin (HE) and Masson’s Trichrome. Data were analyzed using SPSS version 23 for Windows. Results: There was a statistically signifcant diference in fbroplasia (p=0.014; p=0.018) on the ffth and fourteenth days. However, there was no signifcant diference in angiogenesis (p=0.183; p=0.524) or collagenization (p=0.218; p=0.278) on the ffth and fourteenth day. On the ffth day, the number of capillary vessels was highest in the PRP groups (10.60±4.13), and the ratio of type III collagen fbers (53.00±13.00) was the highest in the EGF group. On the fourteenth day, the number of capillaries and the ratio of type III collagen fbers was the highest in the EGF groups (77.00±16.00). Conclusion: EGF greatly increases the speed of macroscopic healing, accelerates fbroplasia, induces angiogenesis, and is also involved in collagen deposition compared to PRP administration, especially when compared to untreated wounds. Keywords: Wound, EGF, PRP, Epithelialization, Collagenization. Cite This Article: Nurmaulinda, D.S., Budi, A.S., Zarasade, L., Utomo, B. 2021. Epidermal Growth Factor (EGF) representing the role of other growth factors contained in Platelet-Rich Plasma (PRP). Bali Medical Journal 10(2): 757-762. DOI: 10.15562/ bmj.v10i2.2524 1 Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia; 2 Department of Public Health, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia; *Corresponding author: Dessyta Sukma Nurmaulinda; Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia; dessyta.sukma.nurmaulinda-2015@ fk.unair.ac.id Received: 2021-06-18 Accepted: 2021-08-02 Published: 2021-08-21 757 Published by Bali Medical Journal Bali Medical Journal (Bali MedJ) 2021, Volume 10, Number 2: 757-762 P-ISSN.2089-1180, E-ISSN: 2302-2914 Open access: www.balimedicaljournal.org INTRODUCTION A wound is a condition where the tissue is broken due to various things. Tis condition is immediately followed by a wound healing process, which is very complex. 1,2 Te use of Epidermal Growth Factor (EGF) and Platelet-Rich Plasma (PRP) is now being developed in the medical feld because wound care with modern dressings that have been widely used takes time to achieve healing. 3,4 EGF can be developed via recombinants and is a product containing a concentration of growth factors. In comparison, PRP is obtained from peripheral blood concentrate. Te advantage of EGF and PRP is that they can be produced in large quantities with minimal efort and their characteristics can be maintained so that they can be used for tissue regeneration. 3,4 EGF is a growth factor that plays a signifcant role in the process of proliferation, cell diferentiation, stimulation of migration, and proliferation of fbroblasts and keratinocytes, encouraging vascular endothelial cell growth and collagen deposition. 5 EGF stimulates keratinocytes, fbroblasts, and endothelial cells in granulation tissue formation. In addition, EGF can stimulate fbroblasts to synthesize Vascular Endothelial Growth Factor (VEGF) and Hepatocyte Growth Factor (HGF). VEGF and HGF are growth factors that play a major role in promoting the angiogenesis process in wounds. Fibroblasts are the primary cells in the dermal layer of the skin that play an important role in the wound healing process and are responsible for various functions such as the secretion of extracellular matrix (ECM) compounds, especially collagen and fbronectin, in addition to remodeling enzymes such as proteases and collagenases. Tus, it is obvious that fbroblasts are the key cells that form the foundation of normal skin. 5-7 PRP plays a role in wound healing with its high content of exogenous growth factors. PRP can release several growth factors in the form of Epidermal Growth Factor (EGF), Platelet-derived Growth Factor (PDGF), Basic Fibroblast Growth Factor (bFGF), Vascular Endothelial Growth Factor (VEGF),