Advances in Biological Research 8 (1): 29-36, 2014 ISSN 1992-006 © IDOSI Publications, 2014 DOI: 10.5829/idosi.abr.2014.8.1.81138 Corresponding Author: Md. Badrul Alam, Department of Pharmacy, Atish Dipankar University of Science and Technology House # 83, Road # 4, Block # B, Banani, Dhaka-1213, Bangladesh. Tel: +88-01717057701. 29 Antioxidant and Anti-Diabetic Activities of the Methanolic Extract of Premna integrifolia Bark Rajib Majumder, Shahina Akter, 1 1 Zannatul Naim, Md. Al-Amin and Md. Badrul Alam 1 2 1,3 Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh 1 Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh 2 Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh 3 Abstract: Premna integrifolia Linn (Family: Verbenaceae) synonyms of Premna serratifolia has tremendous medicinal value. The methanolic extract of Premna integrifolia (MEPI) bark was subjected to investigate its antidiabetic activity along with its in vitro antioxidant activity. Oral glucose tolerance test (OGTT), normoglycemic test and alloxan induced diabetic test was consider for antidiabetic activity evaluation. In addition, total phenolic content, total antioxidant activity, scavenging of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as reducing power assessment was used to evaluate antioxidant potentiality of MEPI. The continuous post treatment for 120 min with the MEPI showed potential hypoglycemic activity in OGTT and normoglycemic rats. At a dose of 300 mg/kg the extract, considerable drop in elevated blood glucose level was observed in the alloxan induced diabetic (p<0.05) rat after 7 days. It revealed radical scavenging properties in the DPPH assay (IC = 8.61 ± 0.16µg/ml). The finding of this study suggested that MEPI provide as a part of 50 scientific support for the use of this species in traditional medicine for Diabetes mellitus. Key words: Premna Integrifolia Diabetes Antioxidant Reducing Power Total Phenolic Content INTRODUCTION Plants play an important role in the treatment of Diabetes mellitus (DM) is an endocrinal metabolic have any modern facilities, normally in developing disorder characterized by chronic hyperglycemia, countries. Report of ethnobotany revealed that about 800 damaged pancreatic cell with carbohydrate medicinal plants have antidiabetic activity [4] and the bio complication, metabolism of fat and protein occurred by active compounds like alkaloids, glycosides, terpenoids, abnormal secretion of insulin, defects in insulin action or carotenoids and floavonoids are very effective drugs both both [1] additionally increased risk of complications of in preclinical and clinical studies [5, 6]. Various side various vascular diseases. Specialist suggested that effects by using insulin and oral hypoglycemic agent, diabetes is the third leading cause of death due to the modern countries and high-tech. pharmaceutical high percentage of morbidity and mortality after cancer companies showed their interest to produce plant base and cardiovascular disorders. For DM various medicine [7]. So, it is important to investigate different complications occurs in human body like renal failure, plants or plant base medicine for DM. On that point of coronary artery disorders, blindness, neurological view we try to figure out the hypoglycemic effect of complications, cerebro-vascular disease, limb amputation, Premna integrifolia on rats. failure of function of different body parts with premature Premna serratifolia Linn (Family: Verbenaceae) death etc [2]. Insulin therapy and life style modifications synonyms of Premna integrifolia, It is known as or long term use of oral hypoglycemic agents, physical “Agnimantha” in Ayurvedic system of medicine, is a exercises with dietary control can be the medication of small-sized tree or a large shrub, up to 9m in height with a Diabetes mellitus [3]. comparatively short trunk and numerous branches. Diabetes, especially who have limited resource and do not