Sumerianz Journal of Medical and Healthcare, 2021, Vol. 4, No. 2, pp. 109-113 ISSN(e): 2663-421X, ISSN(p): 2706-8404 Website: https://www.sumerianz.com DOI: https://doi.org/10.47752/sjmh.42.109.113 © Sumerianz Publication CC BY: Creative Commons Attribution License 4.0 Original Article Open Access *Corresponding Author 109 109 Manufacture of Hard Gelatin Capsules From a Lyophilisate of the Morus Nigra Fruit Sandra Isabel Hernández-González Laboratorio B de Investigación, Facultad de Ciencias Químicas Campus Gómez Palacio, Universidad Juárez del Estado de Durango, Mexico Jesús Iván García-Castañeda Laboratorio B de Investigación, Facultad de Ciencias Químicas Campus Gómez Palacio, Universidad Juárez del Estado de Durango, Mexico José de Jesús Alba-Romero Laboratorio B de Investigación, Facultad de Ciencias Químicas Campus Gómez Palacio, Universidad Juárez del Estado de Durango, Mexico Aurora Martínez-Romero * Laboratorio B de Investigación, Facultad de Ciencias Químicas Campus Gómez Palacio, Universidad Juárez del Estado de Durango, Mexico Email: aurora.martinez@ujed.mx Rodolfo Gerardo Chew-Madinaveitia Instituto Tecnológico de Torreón. Coahuila, Mexico José Luis Ortega-Sanchez Unidad Regional Universitaria de Zonas Áridas. Universidad Autónoma Chapingo, Mexico Article History Received: April 22, 2021 Revised: May 27, 2021 Accepted: June 4, 2021 Published: June 7, 2021 Abstract Morus nigra L. (M. nigra L.) belongs to the Moraceae family. Traditional medicine for its physicochemical properties. To evaluate the physicochemical composition and pharmaceutical stability of hard gelatin capsules of the freeze-dried fruit of M. nigra L. The content of total phenols (CFT) and antioxidant capacity (CA) were evaluated by spectrophotometry, the content of ash, moisture, protein, fat and fiber were determined according to their own standard. The CFT and CA were 25.4 mg gallic acid equivalents/g of sample, and 74.1% inhibition, respectively. On the other hand, the values of ash, moisture, protein, fat and fiber were 4.2%, 7.9%, 3.9%, 0.5% and 3.6%, respectively. Lyophilized M. nigra fruit may be a novel candidate for the development of gelatin hard capsules and other new pharmaceutical products. Keywords: Physicochemical composition; Pharmaceutical stability; M. nigra L. 1. Introduction Morus nigra L. (Moraceae family), also known as blackberry, is one of the most important species of the genus Morus [1].This is native to Iran and belongs to both cultivated and wild mulberry varieties [2].The fruit is in blackberry form and typically black, but sometimes dark blue with a sweet taste [3], and has been used as part of traditional Chinese medicine for a long time [4], because the fruit of M. nigra is rich in compounds phenolics [5], anthocyanins, proteins, and vitamins for urinary incontinence, depression, fever, cancer and diabetes [6]. Among the phenolic compounds it has are gallic acid, ascorbic acid, p-coumaric acid, caffeic acid, to mention some have a great nutritional and organoleptic significance and give the commercial characteristics of the fruits [7]. However, it is difficult to preserve fresh blackberry fruit due to its low acidity. In addition, phenolic compounds are unstable in various solutions and, therefore, it is necessary to coat them to stabilize them, which can be achieved by an encapsulation process [8]. For this reason, there is a real need to apply technologies to improve their useful life, qualities nutritional, organoleptic and health benefits of blackberry fruit to boost commercial production with economic and health benefits [9]. Encapsulation provides a means to control stability, solubility and bioavailability, as well as for the release of bioactive components [10]. On the other hand, stability is the ability to verify the quality of a drug within its specifications. Stability tests must be conducted under conditions that allow information on the stability of the product to be provided in the shortest possible time, for which, the samples must be stored in conditions that accelerate the possible changes that may occur during the validity period; Accelerated stability studies are carried out under exaggerated storage conditions, to increase the rate of chemical, biological degradation or physical changes of a drug [11]. However, there are not yet any studies that show the pharmaceutical stability of freeze-dried capsules of the fruit. M. nigra from the Comarca Lagunera region. Therefore, the purpose of this study was to evaluate and investigate for the first