Received: 14 April 2021 Revised: 10 June 2021 Accepted: 10 June 2021 DOI: 10.1002/ndr2.12019 NEW DISEASE REPORT First report of Hibiscus latent Singapore virus infecting hibiscus in Peninsular Malaysia M.S.M. Yusop 1 Z.A. Mohamed-Hussein 1, 2 H. Bunawan 1 1 Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi Selangor 43600, Malaysia 2 Department of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia Correspondence H. Bunawan, Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi Selangor, 43600, Malaysia. Email: hamidun.bunawan@ukm.edu.my FIGURE 1 Leaf wrinkling symptoms on different individual hibiscus plants Hibiscus rosa-sinensis is an ornamental plant widely cultivated in the tropics and subtropics. The species has been dubbed the queen of trop- ical flowers and can be found in gardens throughout these regions with numerous varieties and hybrids available. The five-petal, red-coloured hibiscus variety is even the national flower of Malaysia, locally called ‘Bunga Raya’ (Chin, 1988). Around March 2019, a disease symptom of leaf wrinkling was observed on the leaves of hibiscus growing near the Faculty of Science and Technology building in Universiti Kebangsaan Malaysia (Figures 1–3). Total RNA was extracted from diseased leaves of 14 individual plants using a QIAGEN RNeasy Plant Mini Kit according to the manu- facturer’s protocol. The RNA extracts were pooled and sent for next- generation sequencing using the Illumina HiSeq 2500 platform (1st BASE Sequencing Malaysia). The extracts were subjected to library This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2021 The Authors. New Disease Reports published by British Society for Plant Pathology and John Wiley & Sons FIGURE 2 Non-symptomatic leaves on different healthy hibiscus plants FIGURE 3 Hibiscus leaf showing wrinkling symptom (left) and a healthy leaf showing no symptoms (right) New Dis Rep. 2021;1–3. wileyonlinelibrary.com/journal/ndr2 1