REV.CHIM.(Bucharest)♦68♦No. 1 ♦2017 http://www.revistadechimie.ro 121 The Study of Analysed Biochemical Parameters on Fruits of Prunus cerasifera Ehrh. Biotypes SINZIANA VENERA MORARITA 1 , ANA MARIA HOSSU 2 *, ANDREEA ANTONIA GEORGESCU 3 1 VALAHIA University of Targoviste, Faculty of Environmental Engineering and Food Science, Department of Environmental Engineering, 18-24, Unirii Str., 130082, Targoviste, Romania 2 VALAHIA University of Targoviste, Faculty of Sciences and Arts, Department of Chemistry, 18-24, Unirii Str., 130082, Targoviste, Romania 3 VALAHIA University of Targoviste, Faculty of Environmental Engineering and Food Science, Department of Food Engineering, 18-24, Unirii Str., 130082, Targoviste, Romania Prunus cerasifera Ehrh. fruits, as well as other stone fruits, have a number of features which make can be used: fresh consumption, in canned food industry and for the manufacture of brandy. In this paper, some fruits of Prunus cerasifera Ehrh. biotypes found in Fruit -Growing Area Voineºti were biochemically analyzed (parameters were: soluble dry matter, vitamin C, titratable acidity expressed in malic acid). The research was carried out over two consecutive years, 2013 and 2014 and 14 biotypes were analyzed: yellow wax cherry 9 and 5 red wax cherry harvested from the area Voinesti (Dambovita). Keywords: Prunus cerasifera Ehrh., soluble dry matter, vitamin C, malic acid Generally, Prunus cerasifera Ehrh. fruits cannot compare with the best fruits for meal neither the taste nor size. Negative feedback that is generally towards this fruits is due to the mix of types and different shapes in terms of their quality and the fact that most often these are harvested raw, unmatured and they are away to have maximum taste qualities. If it left to mature fruits, many of Prunus cerasifera Ehrh. biotypes have acceptable taste qualities, being rich in sugar than some summer plums, others have a more pronounced flavor and quite rich in vitamin C. Often the consumer is attracted only by the size fruit and only second of their wealth in sugar. The industrialization or fruit processing is done in so a limited framework for household needs and in large quantities as a marketable commodity. From Prunus cerasifera Ehrh. fruits are made compotes, jams, marmalade and brandy, sometimes even less juice or wine and dried fruits [1]. In 2013 and 2014, 14 Prunus cerasifera Ehrh. biotypes were harvested and analysed: yellow wax cherry 9 and 5 red wax cherry. The analysed biotypes were harvested from Voinesti area, a region recognized as reference for fruit-grower in our country. For the biotypes of wax cherry to which the fruit analyzes conducted in 2013 to good results in some biophysical or biochemical parameters, in 2014 tests were repeated to see if the values obtained in the previous year are the same or close, allowing to recommend as biotypes with edible or technological superior qualities of fruits. From each biotype, it was necesarry 100 fruits for analyzes. The analyzed biophysical parameters were: total weight of the fruit, pulp weight, kernels weight, height and diameter of fruit. The analyzed biochemical parameters were: titratable acidity expressed in malic acid, soluble dry matter, vitamin C. * email: anahossu@yahoo.co.uk Experimental part The acidity of food can be determined by alkali titration in the presence of phenolphthalein as an indicator. R – COOH + NaOH → R – COONa + H 2 O The reagents used were: - sodium hydroxide, 0.1N; - phenolphthalein solution 1% in ethanol 95% vol. Weigh 10 g of the product, it increases well and places in a 250 mL Erlenmeyer flask with 50 mL of distilled water and stir to mix. The Erlenmeyer flask containing the sample adapted to a reflux condenser and heated on a boiling water bath for 30 min. Cool the content of the flask at room temperature, then decant quantitatively into a 250 mL volumetric flask and make up with distilled water (V1). It is stirred to homogeneity and filtered through a medium-porosity filter paper. Collect 50 mL of the filtrate, it is added several drops of the phenolphthalein solution and titrated with aqueous sodium hydroxide 0.1 N until the appearance of pink color which persists for 30 seconds. Record the volume of sodium hydroxide solution used in the titration of 0.1 N (V3). Carry out two parallel determinations of the same sample for analysis [2]. By soluble substances is meant the concentration, expressed in weight percent of an aqueous solution of sucrose which has the same refractive index as the product analyzed, according to the determination. The method is based on the relationship that exists between the refractive index and the content of soluble substance from a solution. At refractometer, it is reading the refractive index and the percentage of soluble solids at a temperature of 20± 0.5° C. For determinations, it is necessary Abbe refractometer provided with a graduated scale the values of the refractive indices and percentage of sucrose. It makes an average sample of fruit pulp. Squeeze through gauze clean and dry,