Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Fri, 07 Dec 2018 13:25:39 Micromonospora fluostatini sp. nov., isolated from marine sediment Wongsakorn Phongsopitanun, 1 Takuji Kudo, 2 Mihoko Mori, 3,4 Kazuro Shiomi, 3,4 Pattama Pittayakhajonwut, 5 Khanit Suwanborirux 6 and Somboon Tanasupawat 1 Correspondence Somboon Tanasupawat Somboon.T@chula.ac.th 1 Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 2 Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan 3 Graduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan 4 Kitasato Institute for Life Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan 5 National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathumthani 12120, Thailand 6 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand The novel actinomycete strain PWB-003 T , which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003 T represented a member of the genus Micromonospora. It produced single spores on substrate mycelia and contained meso- diaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H 4 ). Cellular fatty acids comprised C 18 : 1 v9c, iso-C 16 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 and iso-C 17 : 0 . On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to Micromonospora eburnea LK2-10 T (99.38 %), Micromonospora chaiyaphumensis MC5-1 T (99.16 %), Micromonospora yangpuensis FXJ6.011 T (98.97 %), Micromonospora echinaurantiaca DSM 43904 T (98.97 %), Micromonospora pallida DSM 43817 T (98.97 %), Micromonospora sagamiensis DSM 43912 T and Micromonospora auratinigra JCM 12357 T (both 98.97 %). The G+C content of the DNA was 74.5 mol%. DNA–DNA relatedness values among strain PWB-003 T and related type strains ranged from 11.3¡1.3 to 38.8¡1.1 %. On the basis of these observations, strain PWB-003 T could be distinguished from its closely related type strains and is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora fluostatini sp. nov. (type strain PWB- 003 T 5JCM 30529 T 5PCU 341 T 5TISTR 2345 T ) is proposed. The genus Micromonospora, accommodating aerobic, Gram- stain-positive, filamentous bacteria that form single non- motile spores on substrate mycelia, was initially described by Ørskov (1923). Although many species of the genus Micromonospora have been isolated from diverse soils col- lected from different regions (Genilloud, 2012), some have been isolated from root nodules (Trujillo et al., 2006), sea sand (Tanasupawat et al., 2010) and marine sponge (Supong et al., 2013). Since discovery of the first antibiotics, more than 740 bioactive metabolites, such as gentamicins, neomycin, rifamycins, sisomicin, erythromycin B and forti- micins, have been isolated from the family Micromonospora- ceae and have been used for clinical treatment (Be ´rdy, 2005; Wagman & Weinstein, 1980). In the present paper, we report the isolation, taxonomy and secondary metabolites of a novel strain, PWB-003 T , belonging to the genus Micromonospora. Abbreviation: gyrB, gyrase B subunit. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and gyrB gene sequences of strain PWB-003 T are LC033898 and LC071513, respectively. Two supplementary tables and five supplementary figures are available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2015), 65, 4417–4423 DOI 10.1099/ijsem.0.000589 000589 G 2015 IUMS Printed in Great Britain 4417